Isotope dilution gas chromatographic-mass spectrometric method for the determination of isoflavonoids, coumestrol, and lignans in food samples
Φόρτωση...
Ημερομηνία
Συγγραφείς
Mazur, W.
Fotsis, T.
Wahala, K.
Ojala, S.
Salakka, A.
Adlercreutz, H.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Anal Biochem
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
We present a method for the quantitative determination of the phytoestrogens formononetin, biochanin A, daidzein, genistein, and coumestrol and simultaneously the lignans secoisolariciresinol (SECO) and matairesinol in plant-derived foods. These compounds are measured by isotope dilution gas chromatography-mass spectrometry in the selected ion monitoring mode (ID/GC/MS/SIM) using synthesized deuterated internal standards for the correction of losses during the procedure. A three-step hydrolysis--a rehydration with distilled H2O, followed by enzymatic and acid hydrolysis--has been applied in order to convert the diphenolic glycosides into their respective aglycones. Purification and separation are carried out in two ion-exchange chromatographic steps followed by derivatization and GC-MS. The within-assay imprecision values vary 3.1-9.6% and the between-assay imprecision 7.0-21.2%. The mean recovery of authentic standards processed through the whole procedure varied from 95.5 to 105.5%. Values for some different food samples are presented. The simultaneous determination of the biologically most interesting phytoestrogens and lignans in foods has not been carried out previously and the method will be useful for screening of important foods in populations with different risk of cancer and coronary heart disease, and for metabolic studies.
Περιγραφή
Λέξεις-κλειδιά
Anion Exchange Resins, Chromatography, Ion Exchange, Coumestrol/*analysis, Flavonoids/*analysis, *Food Analysis, Gas Chromatography-Mass Spectrometry/*methods, Hydrolysis, Lignans/*analysis, Reference Standards, Reproducibility of Results
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/8789715
http://ac.els-cdn.com/S0003269796900251/1-s2.0-S0003269796900251-main.pdf?_tid=dfdcb2bc0f9dc14b01bdc2791862f51e&acdnat=1332752960_404eaf4d30e307f441beb13e6b264255
http://ac.els-cdn.com/S0003269796900251/1-s2.0-S0003269796900251-main.pdf?_tid=dfdcb2bc0f9dc14b01bdc2791862f51e&acdnat=1332752960_404eaf4d30e307f441beb13e6b264255
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής