Cu(II) and Ni(II) interactions with the terminally blocked hexapeptide Ac-Leu-Ala-His-Tyr-Asn-Lys-amide model of histone H2B (80-85)

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dc.contributor.authorPanagiotou, K.en
dc.contributor.authorPanagopoulou, M.en
dc.contributor.authorKaravelas, T.en
dc.contributor.authorDokorou, V.en
dc.contributor.authorHagarman, A.en
dc.contributor.authorSoffer, J.en
dc.contributor.authorSchweitzer-Stenner, R.en
dc.contributor.authorMalandrinos, G.en
dc.contributor.authorHadjiliadis, N.en
dc.date.accessioned2015-11-24T16:55:09Z
dc.date.available2015-11-24T16:55:09Z
dc.identifier.issn1565-3633-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/10262
dc.rightsDefault Licence-
dc.subjectmetal-binding sequenceen
dc.subjectglycyl-l-histidineen
dc.subjectcoordination propertiesen
dc.subjectoxidative damageen
dc.subjection complexesen
dc.subjecteshh-motifen
dc.subjectpeptideen
dc.subjecttailen
dc.subjectcopper(ii)en
dc.subjectresiduesen
dc.titleCu(II) and Ni(II) interactions with the terminally blocked hexapeptide Ac-Leu-Ala-His-Tyr-Asn-Lys-amide model of histone H2B (80-85)en
heal.abstractThe N- and C-terminal blocked hexapeptide Ac-Leu-Ala-His-Tyr-Asn-Lys-amide (LAHYNK) representing the 80-85 fragment of histone H2B was synthesized and its interactions with Cu(II) and Ni(II) ions were studied by potentiometric, UV-Vis, CD, EPR, and NMR spectroscopic techniques in solution. Our data reveal that the imidazole N(3) nitrogen atom is the primary ligating group for both metal ions. Sequential amide groups deprotonation and subsequent coordination to metal ions indicated an {N(imidazole), 3N(amide)} coordination mode above pH similar to 9, in all cases. In the case of Cu(II)-peptide system, the almost exclusive formation of the predominant species CuL in neutral media accounting for almost 98% of the total metal ion concentration at pH 7.3 strongly indicates that at physiological pH values the sequence -LAHYNK- of histone H2B provides very efficient binding sites for metal ions. The imidazole pyrrole N(1) ionization (but not coordination) was also detected in species CuH-4L present in solution above pH similar to 11. Copyright (C) 2008 Katerina Panagiotou et al.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDoi 10.1155/2008/257038-
heal.identifier.secondary<Go to ISI>://000255639900001-
heal.identifier.secondaryhttp://downloads.hindawi.com/journals/bca/2008/257038.pdf-
heal.journalNameBioinorg Chem Applen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2008-
heal.publisherHindawi Publishing Corporationen
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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