Down-regulation of endothelial cell growth inhibitors by enhanced MYCN oncogene expression in human neuroblastoma cells
Φόρτωση...
Ημερομηνία
Συγγραφείς
Fotsis, T.
Breit, S.
Lutz, W.
Rossler, J.
Hatzi, E.
Schwab, M.
Schweigerer, L.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Eur J Biochem
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Recent evidence indicates that the genetic alterations of the multistage process of malignant transformation appear to activate tumor neovascularization by altering the balance between stimulators and inhibitors of angiogenesis. In the present study, we have attempted to define the effect of enhanced MYCN oncogene expression on the profile of endothelial cell growth modulators in neuroblastoma cells. We report here that conditioned medium of human neuroblastoma cells with normal MYCN expression contains three inhibitors of endothelial cell proliferation, which appear to be novel proteins as judged by their physicochemical, immunological and biological properties. All three inhibitors are diminished or become undetectable upon experimental increase of MYCN expression. Our results suggest that enhanced MYCN expression in human neuroblastoma cells alters the angiogenic balance by down-regulating endothelial cell growth inhibitors but leaving the expression of the stimulators unaffected. These data shed light on the molecular mechanisms linking the genetic changes of malignant transformation with initiation of tumor angiogenesis. Moreover, our observations might explain the poor prognosis of human neuroblastomas following MYCN oncogene amplification through initiation of angiogenesis and subsequent tumor growth and spread.
Περιγραφή
Λέξεις-κλειδιά
Cell Division/drug effects, Cell Transformation, Neoplastic, Cells, Cultured, Chromatography, Affinity, Culture Media, Conditioned, Endothelium, Vascular/cytology/drug effects/*physiology, Fibroblast Growth Factor 2/*pharmacology, *Gene Expression Regulation, Neoplastic, *Genes, myc, Growth Inhibitors/*biosynthesis/isolation & purification, Humans, Neovascularization, Pathologic, Neuroblastoma, Proto-Oncogene Proteins c-myc/*genetics, Recombinant Proteins/pharmacology, Tumor Cells, Cultured
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/10469139
http://onlinelibrary.wiley.com/store/10.1046/j.1432-1327.1999.00575.x/asset/j.1432-1327.1999.00575.x.pdf?v=1&t=h099l0t9&s=a72c9723587e2264dfef85bea3a0e262bf6cd2e5
http://onlinelibrary.wiley.com/store/10.1046/j.1432-1327.1999.00575.x/asset/j.1432-1327.1999.00575.x.pdf?v=1&t=h099l0t9&s=a72c9723587e2264dfef85bea3a0e262bf6cd2e5
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής