A model to study total hepatic ischemia-reperfusion injury
| dc.contributor.author | Kanoria, S. | en |
| dc.contributor.author | Glantzounis, G. | en |
| dc.contributor.author | Jalan, R. | en |
| dc.contributor.author | Davies, N. A. | en |
| dc.contributor.author | Seifalian, A. M. | en |
| dc.contributor.author | Williams, R. | en |
| dc.contributor.author | Davidson, B. R. | en |
| dc.date.accessioned | 2015-11-24T19:40:15Z | |
| dc.date.available | 2015-11-24T19:40:15Z | |
| dc.identifier.issn | 0041-1345 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/24323 | |
| dc.rights | Default Licence | - |
| dc.subject | Alanine Transaminase/blood | en |
| dc.subject | Animals | en |
| dc.subject | Aspartate Aminotransferases/blood | en |
| dc.subject | Blood Pressure | en |
| dc.subject | Disease Models, Animal | en |
| dc.subject | Kinetics | en |
| dc.subject | L-Lactate Dehydrogenase/blood | en |
| dc.subject | *Liver Circulation | en |
| dc.subject | Oxygen/blood | en |
| dc.subject | Rabbits | en |
| dc.subject | Reperfusion Injury/enzymology/*physiopathology | en |
| dc.subject | Time Factors | en |
| dc.title | A model to study total hepatic ischemia-reperfusion injury | en |
| heal.abstract | BACKGROUND: Most experimental animal models for studying hepatic ischemia-reperfusion injury (IRI) involve partial or segmental ischemia of the liver or a portocaval shunt procedure to avoid mesenteric congestion. However, these do not reflect the global ischemia that occurs during liver transplantation. A rabbit model of total hepatic ischemia without a portocaval shunt is described. METHODS: Twenty male New Zealand white rabbits (3.5 +/- 0.3 kg) were allocated to four groups: group 1 (n = 5), sham-operated; group 2 (n = 5), 20-minute total hepatic ischemia; group 3 (n = 5), 25-minute total hepatic ischemia; and group 4 (n = 5), 30-minute total hepatic ischemia. Total hepatic ischemia was induced by occluding the portal inflow vessels (portal vein and artery) with an atraumatic vascular loop and were measurements taken for 2 hours during reperfusion. RESULTS: A total hepatic ischemia of 30 minutes caused severe liver injury resulting in cardiac arrest at 2 hours of reperfusion in all five animals due to metabolic acidosis. Twenty minutes of total ischemia was tolerated and did not produce significant liver injury. Twenty-five minutes of total ischemia was tolerated but at 2 hours of reperfusion, resulted in significant liver injury (68 +/- 41, 283.0 +/- 20.5, and 835.2 +/- 52.7 U/L) compared with the sham-operated group (serum ALT, 25.4 +/- 2.7; serum AST, 47.4 +/- 3.0; serum LDH, 307.6 +/- 44.4 U/L; P < .003). CONCLUSIONS: Rabbits can tolerate 25 minutes of total hepatic ischemia without a portosystemic shunt. This 25-minute ischemia model simulates operative conditions during liver transplantation and will be valuable in studies modulating IRI. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | 10.1016/j.transproceed.2004.10.031 | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/15621096 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0041134504011972/1-s2.0-S0041134504011972-main.pdf?_tid=4affcdb7f891cb36ec11835fc9a3dfe7&acdnat=1333626340_1f4056c21374cd3b58080d05b3a1f500 | - |
| heal.journalName | Transplant Proc | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2004 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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