Complementary segmental labeling of large RNAs: economic preparation and simplified NMR spectra for measurement of more RDCs
| dc.contributor.author | Tzakos, A. G. | en |
| dc.contributor.author | Easton, L. E. | en |
| dc.contributor.author | Lukavsky, P. J. | en |
| dc.date.accessioned | 2015-11-24T16:54:12Z | |
| dc.date.available | 2015-11-24T16:54:12Z | |
| dc.identifier.issn | 0002-7863 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/10128 | |
| dc.rights | Default Licence | - |
| dc.subject | Carbon Isotopes | en |
| dc.subject | Imines/chemistry | en |
| dc.subject | Isotope Labeling/*methods | en |
| dc.subject | Magnetic Resonance Spectroscopy/methods | en |
| dc.subject | Nitrogen Isotopes | en |
| dc.subject | Nucleic Acid Conformation | en |
| dc.subject | Pharmaceutical Preparations/*chemical synthesis/*economics | en |
| dc.subject | RNA/*chemistry | en |
| dc.subject | Transcription, Genetic | en |
| dc.title | Complementary segmental labeling of large RNAs: economic preparation and simplified NMR spectra for measurement of more RDCs | en |
| heal.abstract | NMR structure determination of large RNAs is often restricted by limited RDC information caused by chemical shift degeneracy. We established a general, time- and cost-effective methodology for the preparation of 13C/15N complementary labeled RNAs from a single plasmid. Applying this method to the 25 kDa BC1-DTE RNA, we were able to resolve severe chemical shift degeneracy, thereby almost doubling the number of RDC restraints in comparison to the conventional 13C,15N uniform-labeled RNA. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | 10.1021/ja064807o | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/17031932 | - |
| heal.identifier.secondary | http://pubs.acs.org/doi/pdfplus/10.1021/ja064807o | - |
| heal.journalName | J Am Chem Soc | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2006 | - |
| heal.publisher | American Chemical Society | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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