Collagen models as a probe in the decay of works of art: synthesis, conformation and immunological studies
Φόρτωση...
Ημερομηνία
Συγγραφείς
Zevgiti, S.
Sakarellos, C.
Sakarellos-Daitsiotis, M.
Ioakimoglou, E.
Panou-Pomonis, E.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Wiley
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Peptide Science
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Proteinaceous substances such as collagen, casein and albumin have been widely used as binding media in a variety of works of art. Damages of these 'sensitive' materials, mainly caused of the influence of the environment, are responsible for the overall decay of works of art, and their identification is essential to understand ancient technologies, determine the extent of deterioration and help in future restoration and preservation processes. The most commonly used techniques for the identification of proteinaceous binding media are staining techniques, chromatography, spectrometry and immunological methods, although for the latter no systematic studies have been carried out until now. Aiming at contributing to the development of a reliable and reproducible immunoassay for the evaluation of the collagen-based decay of works of art, sequential polypeptides (Pro-X-Gly)(n) where X represents amino acid residues Val, Lys, Glu and (Hyp-Val-Gly)(n) were prepared as models of collagen fragments derived from artificially and naturally aged animal collagens. Conformational studies of the polypeptides by CID revealed the occurrence of polyproline II-like structures comparable with those of collagen. Polypeptides and collagen I were administered to animals, and the induced antibodies were used for the immunochemical detection and differentiation of collagen and collagen fragments. The combined application of (i) anti-collagen antibodies, which strongly interact with native collagen, but poorly recognized by artificially aged collagen and (ii) anti- polypeptide antibodies, which do not associate with native collagen, but are strongly recognized by collagen fragments in naturally or artificially aged collagen, is a valuable tool in determining the extent of decay in works of art. Copyright (c) 2006 European Peptide Society and John Wiley & Sons, Ltd.
Περιγραφή
Λέξεις-κλειδιά
collagen model peptides, circular dichroism, immunological assays, artificial ageing of collagen, decay of works of art, n-isobutylglycine nleu, triple-helix, sequential polypeptides, circular-dichroism, optical-rotation, ultraviolet absorbency, repeating sequence, binding media, amino-acids, peptides
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000244370900007
http://onlinelibrary.wiley.com/store/10.1002/psc.823/asset/823_ftp.pdf?v=1&t=h0e0wf97&s=cd9afc166ca079b2bf7e4b09231b5e425734659d
http://onlinelibrary.wiley.com/store/10.1002/psc.823/asset/823_ftp.pdf?v=1&t=h0e0wf97&s=cd9afc166ca079b2bf7e4b09231b5e425734659d
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας