Studies on glycogen autophagy: effects of phorbol myristate acetate, ionophore A23187, or phentolamine
Φόρτωση...
Ημερομηνία
Συγγραφείς
Kalamidas, S. A.
Kotoulas, O. B.
Hann, A. C.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Microsc Res Tech
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The effects of agents that could manipulate the lysosomal calcium such as phorbol myristate acetate, ionophore A23187, and phentolamine on the lysosomal glycogen degradation were studied by electron microscopy, morphometric analysis, and biochemical assays in newborn rat hepatocytes. Phorbol myristate acetate, which promotes the input of calcium to lysosomes, increased the total volume of autophagic vacuoles and the activity of lysosomal glycogen-hydrolyzing acid alpha 1,4 glucosidase and decreased the fractional volume of undigested glycogen inside the autophagic vacuoles and also decreased the activity of acid mannose 6-phosphatase. Ionophore A23187, which releases lysosomal calcium, produced opposite results in these enzyme activities. Phentolamine, an alpha-adrenergic blocking agent which interferes with the generation of phosphoinositides and may activate the lysosomal calcium uptake pump, increased the total volume of autophagic vacuoles and the activity of lysosomal glycogen-hydrolyzing acid glucosidase and decreased the fractional volume of undigested glycogen inside the autophagic vacuoles. The results of this study constitute evidence that changes in lysosomal calcium may influence certain aspects of autophagy, including the degradation of glycogen inside the autophagic vacuoles. They also support our previous postulate [Kalamidas and Kotoulas (2000a,b) Histol Histopathol 15:29-35, 1011-1018] that stimulation of autophagic mechanisms in newborn rat hepatocytes may be associated with acid mannose 6-phosphatase activity-deficient lysosomes.
Περιγραφή
Λέξεις-κλειδιά
Animals, Animals, Newborn, Autophagy/*drug effects, Calcimycin/administration & dosage/*pharmacology, Cells, Cultured, Cyclic AMP/administration & dosage, Glycogen/*metabolism, Hepatocytes/drug effects/metabolism/ultrastructure, Ionophores/administration & dosage/*pharmacology, Liver/cytology, Lysosomes/metabolism, Microscopy, Electron, Phentolamine/administration & dosage/*pharmacology, Rats, Rats, Wistar, Tetradecanoylphorbol Acetate/*pharmacology
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/12112433
http://onlinelibrary.wiley.com/store/10.1002/jemt.10104/asset/10104_ftp.pdf?v=1&t=h1ym158g&s=4ab08745ec77a77df13f251dea2ed35f9f599948
http://onlinelibrary.wiley.com/store/10.1002/jemt.10104/asset/10104_ftp.pdf?v=1&t=h1ym158g&s=4ab08745ec77a77df13f251dea2ed35f9f599948
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής