Fine needle aspiration biopsy-RT-PCR molecular analysis of thyroid nodules: a useful preoperative diagnostic tool
| dc.contributor.author | Letsas, K. P. | en |
| dc.contributor.author | Andrikoula, M. | en |
| dc.contributor.author | Tsatsoulis, A. | en |
| dc.date.accessioned | 2015-11-24T19:15:46Z | |
| dc.date.available | 2015-11-24T19:15:46Z | |
| dc.identifier.issn | 0391-1977 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/21560 | |
| dc.rights | Default Licence | - |
| dc.subject | Biopsy, Fine-Needle/*methods | en |
| dc.subject | Humans | en |
| dc.subject | *Preoperative Care | en |
| dc.subject | *Reverse Transcriptase Polymerase Chain Reaction | en |
| dc.subject | Sensitivity and Specificity | en |
| dc.subject | Sequence Analysis, DNA | en |
| dc.subject | Thyroid Nodule/*diagnosis/*genetics/surgery | en |
| dc.subject | Thyroidectomy | en |
| dc.title | Fine needle aspiration biopsy-RT-PCR molecular analysis of thyroid nodules: a useful preoperative diagnostic tool | en |
| heal.abstract | Fine needle aspiration biopsy (FNAB)-based cytology is an accurate preoperative diagnostic method for the evaluation of thyroid nodules. Despite its high specificity and sensitivity, the results are non-diagnostic in a significant number of cases, commonly due to inadequacy of the samples or the presence of undetermined cellular morphology. Overlap-ping cytologic features among follicular-derived lesions are the most common causes of cyto-histopathologic discordances. The management of thyroid nodules with a non-diagnostic FNAB remains controversial. The significant probability of malignancy imposes either the repeat of the procedure or direct surgery. Recent studies have demonstrated the important role of molecular-based techniques such as RT-PCR in the diagnosis of thyroid lesions. Follicular cells obtained from the remaining material within the needle can be used for RNA extraction and then for RT-PCR amplification of specific mRNAs that are expressed in benign or malignant thyroid nodules. Furthermore, FNAB-RT-PCR in combination with molecular scanning techniques or direct sequencing provides a valuable screening tool for mutations. The present review highlights the applications of FNAB-RT-PCR method in the identification of new diagnostic molecular markers. The parallel molecular and cytological analysis of FNABs may contribute to a more accurate diagnosis and, thus, to a more specific clinical management of thyroid nodules. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/16682941 | - |
| heal.journalName | Minerva Endocrinol | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2006 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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