Protein stability in nanocages: a novel approach for influencing protein stability by molecular confinement
Φόρτωση...
Ημερομηνία
Συγγραφείς
Bolis, D.
Politou, A. S.
Kelly, G.
Pastore, A.
Temussi, P. A.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
J Mol Biol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Confinement of a protein in a small inert space and microviscosity are known to increase its thermodynamic stability in a way similar to the mechanisms that stabilize protein fold in the cell. Here, to examine the influence of confinement on protein stability we choose four test cases of single domain proteins characterized by a wide range of melting temperatures, from approximately 73 degrees C of titin I27 to approximately 36 degrees C of yeast frataxin. All proteins are stabilized when confined in the gel, the most dramatic stabilization being that of yeast frataxin, whose melting temperature increased by almost 5 degrees C in the gel. In addition to being simple to use, this approach allows us to change the viscosity of the solvent without changing its composition or altering the structure of the proteins. The dimensions of the pores of the gels fall in the nanometer range, hence they are similar to those of the chaperone cavity. This method could therefore be used as a novel and powerful approach for protein folding studies.
Περιγραφή
Λέξεις-κλειδιά
Acrylic Resins/*chemistry, Bacterial Proteins/*chemistry/metabolism, Escherichia coli Proteins, Humans, Iron-Binding Proteins/*chemistry/metabolism, Muscle Proteins/*chemistry/metabolism, *Protein Conformation, Protein Denaturation, Protein Folding, Protein Kinases/*chemistry/metabolism, Temperature, Thermodynamics, Viscosity
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/14741216
http://ac.els-cdn.com/S002228360301475X/1-s2.0-S002228360301475X-main.pdf?_tid=afa6bd12c920aefeb664b5b9311e5fc2&acdnat=1333004702_c19eb14f82394495851559569a953dca
http://ac.els-cdn.com/S002228360301475X/1-s2.0-S002228360301475X-main.pdf?_tid=afa6bd12c920aefeb664b5b9311e5fc2&acdnat=1333004702_c19eb14f82394495851559569a953dca
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής