Methylation status of the SNRPN and HUMARA genes in testicular biopsy samples
Φόρτωση...
Ημερομηνία
Συγγραφείς
Dasoula, A.
Georgiou, I.
Kontogianni, E.
Sofikitis, N.
Syrrou, M.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Fertil Steril
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
OBJECTIVE: To analyze the methylation status of two differentially inherited and methylated loci (the human androgen receptor [HUMARA] and the small nuclear ribonucleoprotein-associated polypeptide N [SNRPN] gene) in testicular biopsy samples, and to compare the results with microscopic evaluation. DESIGN: Retrospective study. SETTING: Infertility clinics and genetics laboratories. PATIENT(S): Twelve obstructive and 74 nonobstructive azoospermic men. INTERVENTION(S): Deoxyribonucleic acid samples from testicular biopsies and peripheral blood were modified with sodium bisulfite and amplified by methylation-specific polymerase chain reaction assay. Polymerase chain reaction primers specific for the methylated regions of the HUMARA locus and for the methylated and unmethylated CpG islands of the SNRPN gene were used. MAIN OUTCOME MEASURE: Polymerase chain reaction product bands specific for methylated and unmethylated alleles. RESULT(S): Obstructive azoospermia patients were positive for spermatozoa and germ cells by all approaches (microscopic, HUMARA, and SNRPN analysis) with absolute consistency. In contrast, for the nonobstructive men, microscopy was consistent with SNRPN analysis as regards the presence of germ cells in 82% of the testicular tissues tested. Nonobstructive patients with maturation arrest were positive for the presence of germ cells only by HUMARA analysis, with 84% sensitivity. CONCLUSION(S): Methylation analysis of testicular tissue is consistent with microscopic analysis, in terms of the prevalence of germ cells and the stage of spermatogenic arrest in biopsy samples.
Περιγραφή
Λέξεις-κλειδιά
Adult, Autoantigens/*genetics, Azoospermia/*genetics, Biopsy, CpG Islands, *DNA Methylation, Humans, Male, Receptors, Androgen/*genetics, Retrospective Studies, Ribonucleoproteins, Small Nuclear/*genetics, Testis/*metabolism/pathology, snRNP Core Proteins
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/17207798
http://ac.els-cdn.com/S0015028206043858/1-s2.0-S0015028206043858-main.pdf?_tid=a4317bb9bcf2a2592d7919cbba51dc52&acdnat=1333716574_5d8d93acb907d56f3346e17c025709c3
http://ac.els-cdn.com/S0015028206043858/1-s2.0-S0015028206043858-main.pdf?_tid=a4317bb9bcf2a2592d7919cbba51dc52&acdnat=1333716574_5d8d93acb907d56f3346e17c025709c3
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής