Novel chemiluminescence method for detection of superoxide anions and its application to dry-cured meat

dc.contributor.authorYao, D. C.en
dc.contributor.authorVlessidis, A. G.en
dc.contributor.authorEvmiridis, N. P.en
dc.contributor.authorZhou, Y. K.en
dc.contributor.authorXu, S. Q.en
dc.contributor.authorZhou, H. R.en
dc.date.accessioned2015-11-24T16:42:51Z
dc.date.available2015-11-24T16:42:51Z
dc.identifier.issn0003-2670-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/8609
dc.rightsDefault Licence-
dc.subjectsuperoxide anionsen
dc.subjectchemiluminescenceen
dc.subjectluminolen
dc.subjectdry-cured meaten
dc.subjectnitric-oxide synthaseen
dc.subjectendothelial-cellsen
dc.subjectdismutase activityen
dc.subjectascorbic-aciden
dc.subjectluminescenceen
dc.subjectinflammationen
dc.subjectoxygenen
dc.subjectironen
dc.subjectdehydrogenaseen
dc.subjectfluorescenceen
dc.titleNovel chemiluminescence method for detection of superoxide anions and its application to dry-cured meaten
heal.abstractA novel method for detection of superoxide anions (O-2(circle-)) is developed through a luminol-enhanced chemiluminescence (CL) reaction. The superoxide anions in different tissues of rats are extracted with dimethyl sulfoxide and tetrabutylammonium chloride (DMSO-TBAC) solution, and assayed by CL detection. Background or other interference due to other reactive oxygen species (ROS) is minimized and the CL emission conditions are optimized. This system is used to evaluate the levels of superoxide anions in rats that were fed large amounts of dry-cured meat in China. The possible concentration Of O-2(circle-) in the rat tissues is evaluated in the range of 10(-8) to 10(-7) M. The results showed that rats belonging to the dry-cured meat group have much higher levels of O-2(circle-) than the control group, which could lead to tissue damage, such as inflammation and especially acute gingivitis when the inflammation occurs in the mouth. (C) 2002 Elsevier Science B.V. All rights reserved.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDoi 10.1016/S0003-2670(02)00099-5-
heal.identifier.secondary<Go to ISI>://000177899000016-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0003267002000995/1-s2.0-S0003267002000995-main.pdf?_tid=9c1de202-356a-11e3-a54e-00000aacb35e&acdnat=1381821955_a3bfb4a2efb567df5177cdfff5d9d7f7-
heal.journalNameAnalytica Chimica Actaen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2002-
heal.publisherElsevier Massonen
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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