Effect of various chemicals on the aldehyde dehydrogenase activity of the rat liver cytosol
Φόρτωση...
Ημερομηνία
Συγγραφείς
Marselos, M.
Vasiliou, V.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Chem Biol Interact
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The cytosolic activity of aldehyde dehydrogenase (ALDH) was studied in the rat liver, after acute administration of various carcinogenic and chemically related compounds. Male Wistar rats were treated with 27 different chemicals, including polycyclic aromatic hydrocarbons, aromatic amines, nitrosamines, azo dyes, as well as with some known direct-acting carcinogens. The cytosolic ALDH activity of the liver was determined either with propionaldehyde and NAD (P/NAD), or with benzaldehyde and NADP (B/NADP). The activity of ALDH remained unaffected after treatment with 1-naphthylamine, nitrosamines and also with the direct-acting chemical carcinogens tested. On the contrary, polycyclic aromatic hydrocarbons, polychlorinated biphenyls (Arochlor 1254) and 2-naphthylamine produced a remarkable increase of ALDH. In general, the response to the effectors was disproportionate between the two types of enzyme activity, being much in favour for the B/NADP activity. This fact resulted to an inversion of the ratio B/NADP vs. P/NAD, which under constitutive conditions is lower than 1. In this respect, the most potent compounds were found to be polychlorinated biphenyls, 3-methylcholanthrene, benzo(a)pyrene and 1,2,5,6-dibenzoanthracene. Our results suggest that the B/NADP activity of the soluble ALDH is greatly induced after treatment with compounds possessing aromatic ring(s) in their molecule. It is not known, if this response of the hepatocytes is related with the process of chemical carcinogenesis.
Περιγραφή
Λέξεις-κλειδιά
Aldehyde Dehydrogenase/*metabolism, Aldehydes/metabolism, Amines/pharmacology, Animals, Benzaldehydes/metabolism, Carcinogens/*pharmacology, Cytosol/enzymology, Liver/drug effects/*enzymology, NAD/metabolism, NADP/metabolism, Nitrosamines/pharmacology, Oxidation-Reduction, Polycyclic Compounds/pharmacology, Rats, Rats, Inbred Strains, Structure-Activity Relationship
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/2060039
http://ac.els-cdn.com/000927979190054B/1-s2.0-000927979190054B-main.pdf?_tid=6346e68805df2bb8c2e7107b905d81ea&acdnat=1332744708_7b02a2e9b38539b6a6062446084bb970
http://ac.els-cdn.com/000927979190054B/1-s2.0-000927979190054B-main.pdf?_tid=6346e68805df2bb8c2e7107b905d81ea&acdnat=1332744708_7b02a2e9b38539b6a6062446084bb970
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής