Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water-organic solvent mixtures
Φόρτωση...
Ημερομηνία
Συγγραφείς
Topakas, E.
Stamatis, H.
Biely, P.
Kekos, D.
Macris, B. J.
Christakopoulos, P.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Biotechnology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
An extracellular feruloyl esterase (FAE-II) from the culture filtrates of Fusarium oxysporum F3 was purified to homogeneity by SP-Sepharose, t-butyl-HIC and Sephacryl S-200 column chromatography. The protein corresponded to molecular mass and pI values of 27 kDa and 9.9, respectively. The enzyme was optimally active at pH 7 and 45 degreesC. The purified esterase was fully stable at pH 7.0-9.0 and temperature up to 45 degreesC after 1 h incubation. Determination of k(cat)/K-m revealed that the enzyme hydrolysed methyl sinapinate 6, 21 and 40 times more efficiently than methyl ferulate, methyl coumarate and methyl caffeate, respectively. The enzyme was active on substrates containing ferulic acid ester linked to the C-5 but inactive to the C-2 positions of arabinofuranose such as 4-nitrophenyl 5-O-transferuloyl-alpha-L-arabinofuranoside and 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside. In the presence of Sporotrichum thermophile xylanase, there was a significant release of ferulic acid from destarched wheat bran by FAE-II, indicating a synergistic interaction between FAE-II and S. thermophile xylanase. FAE-II by itself could release only little ferulic acid from destarched wheat bran. The potential of FAE-II for the synthesis of various phenolic acid esters was tested using as a reaction system a surfactantless microemulsion formed in ternary mixture consisting of n-hexane, 1-propanol and water. (C) 2003 Elsevier Science B.V. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
fusarium oxysporum, feruloyl esterase, purification, esterification, microemulsions, primary-cell wall, aspergillus-niger, wheat bran, detergentless microemulsions, enzymatic-synthesis, fae-iii, release, lipase, polysaccharides, antioxidants
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000182299400004
http://ac.els-cdn.com/S0168165602003632/1-s2.0-S0168165602003632-main.pdf?_tid=925cae66c6aebc1515dcb058694d368c&acdnat=1335509962_e53393034142162d3c088ec3eacae592
http://ac.els-cdn.com/S0168165602003632/1-s2.0-S0168165602003632-main.pdf?_tid=925cae66c6aebc1515dcb058694d368c&acdnat=1335509962_e53393034142162d3c088ec3eacae592
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών