Prenatal paternity testing using DNA extracted from coelomic cells

dc.contributor.authorMakrydimas, G.en
dc.contributor.authorGeorgiou, I.en
dc.contributor.authorKranas, V.en
dc.contributor.authorKaponis, A.en
dc.contributor.authorLolis, D.en
dc.date.accessioned2015-11-24T18:57:01Z
dc.date.available2015-11-24T18:57:01Z
dc.identifier.issn1015-3837-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19125
dc.rightsDefault Licence-
dc.subjectApolipoproteins B/geneticsen
dc.subjectChorion/cytologyen
dc.subjectChorionic Villi Samplingen
dc.subjectDNA/*analysisen
dc.subjectFemaleen
dc.subjectGestational Ageen
dc.subjectHumansen
dc.subjectIodide Peroxidase/geneticsen
dc.subject*Paternityen
dc.subjectPolymerase Chain Reactionen
dc.subjectPregnancyen
dc.subjectPrenatal Diagnosis/*methodsen
dc.titlePrenatal paternity testing using DNA extracted from coelomic cellsen
heal.abstractOBJECTIVE: Prenatal paternity testing can be performed following invasive prenatal diagnosis with amniocentesis or CVS. Coelocentesis is a new technique that could be used as an alternative method early in the first trimester of pregnancy. The aim of this study is to investigate the potential use of the DNA extracted from coelomic cells in the prenatal paternity testing. METHODS: Coelocentesis was performed in 20 singleton pregnancies at 7-9 weeks of gestation immediately before surgical termination of pregnancy. Chorionic cells from the placenta and blood cells from the parents were processed by the standard salt extraction method. Two loci, TPO and Apo B, were used for paternity testing in the DNA of coelomic cells, chorionic cells and blood cells. RESULTS: There was concordance in the results obtained from the coelomic cells and chorionic villi. In two cases only the polymorphisms used were not conclusively informative for paternity exclusion. CONCLUSIONS: Coelomic cells are potentially useful for early paternity testing.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1159/000074265-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/14646423-
heal.identifier.secondaryhttp://content.karger.com/ProdukteDB/produkte.asp?doi=10.1159/000074265-
heal.journalNameFetal Diagn Theren
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2004-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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