Fasciola gigantica cathepsin L proteinase-based synthetic peptide for Immunodiagnosis and prevention of sheep fasciolosis
Φόρτωση...
Ημερομηνία
Συγγραφείς
Jezek, J.
El Ridi, R.
Salah, M.
Wagih, A.
Aziz, H. W.
Tallima, H.
El Shafie, M. H.
Khalek, T. A.
Ammou, F. F. A.
Strongylis, C.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Biopolymers
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Sheep fasciolosis is a devastating burden for the livestock industry. We herein report on immunodiagnosis of fasciolosis, and significant protection of sheep against challenge infection with Fasciola gigantica following immunization with a peptide based on the H-Asp(110)-Lys-Ile-Asp- Trp-Arg-Glu-Ser-Gly-Tyr-Val-Thr-Glu-Val(123)- OH (Fas14p) sequence of E gigantica cathepsin L-cysteine proteinase. This sequence was synthesized in three difterent forms: as N-alpha acetylated (Ac-Asp(110)-Lys-Ile-Asp- Trp-Arg-Glu-Ser-Gly-Tyr-Val-Thr-Glu-Val(123)- OH, FasAc14p), bearing at the amino-terminus an N-alpha acetylated cystein (Ac-Cys-Asp(110)-Lys-Ile-Asp-Trp-Arg-Glu-Ser-Gly- Tyr-Val-Thr-Glu-Val(123)-OH, FasAcCys14p), and conjugated to sequential oligopeptide carrier Ac-[Lys-Aib-Gly](4)-OH (Ac-SOC4) through an amide bond formed between Val(123) carboxylic group of the epitope and the lysine ff groups of the carrier (Ac[Lys(Fas14p)-Aib-GlY](4)-OH). Ac-[Lys(Fas14p)-Aib-Gly](4)-OH failed to readily discriminate between naive and infected sheep. In contrast, the free peptides reproducibly differentiated between parasite-free sheep, sheep infected with parasites other than Fasciola, and experimentally Fasciola-infected sheep. The data together indicated that the peptides might be of considerable use for discriminating between early and late, and low and high burden, sheep infection with F. gigantica. FasAc14p was chosen to determine whether a peptide based on a critical enzymatic site of cathepsin L proteinase may induce protection against challenge infection. Sheep immunization with FasAc14p peptide induced significant expression of interleukin-4 mRNA, and humoral antibodies that bound to molecule(s) on the intact surface membrane of newly excysted juvenile worms, and mediated their attrition. The immune responses were associated with significant (P < 0.02) decrease of 23.1% in worm recovery, but with no decrease in the size or maturation of worms recovered. (c) 2007 Wiley Periodicals, Inc.
Περιγραφή
Λέξεις-κλειδιά
cathepsin l proteinase, peptides, sequential, oligopeptide carriers, synthetic carriers, synthetic peptide, vaccine, fasciola gigantica, immunogenicity, protective potential, liver fluke vaccines, in-vitro, cysteine proteinase, antigenic peptides, hepatica, vaccination, diagnosis, design, cattle, cytotoxicity
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000255448700030
http://onlinelibrary.wiley.com/store/10.1002/bip.20788/asset/20788_ftp.pdf?v=1&t=h0f8nn5b&s=3db456bc323c196d84752e1cc7402aa80d175fa7
http://onlinelibrary.wiley.com/store/10.1002/bip.20788/asset/20788_ftp.pdf?v=1&t=h0f8nn5b&s=3db456bc323c196d84752e1cc7402aa80d175fa7
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας