An in vivo study on the synchronizing effect of hydroxyurea
| dc.contributor.author | Maurer-Schultze, B. | en |
| dc.contributor.author | Siebert, M. | en |
| dc.contributor.author | Bassukas, I. D. | en |
| dc.date.accessioned | 2015-11-24T19:36:30Z | |
| dc.date.available | 2015-11-24T19:36:30Z | |
| dc.identifier.issn | 0014-4827 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23890 | |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | Autoradiography | en |
| dc.subject | Carbon Radioisotopes | en |
| dc.subject | Cell Cycle/*drug effects | en |
| dc.subject | Cell Division/drug effects | en |
| dc.subject | DNA Replication/*drug effects | en |
| dc.subject | Female | en |
| dc.subject | Hydroxyurea/*pharmacology | en |
| dc.subject | Kinetics | en |
| dc.subject | Leukemia L1210/*pathology | en |
| dc.subject | Mice | en |
| dc.subject | Mice, Inbred Strains | en |
| dc.subject | Thymidine/metabolism | en |
| dc.subject | Tritium | en |
| dc.title | An in vivo study on the synchronizing effect of hydroxyurea | en |
| heal.abstract | The effect of hydroxyurea (HU; 0.5 mg/g body wt) on L 1210 ascites tumor cells has been studied using various cell kinetic methods. In contrast to the general assumption that HU blocks cells at the G1/S boundary [J. Brachet (1985) Molecular Cytology, Vol. I, p. 266, Academic Press, New York], the present results show that the cells are not held at G1/S but enter S at about the normal rate and are accumulated in early S phase due to a dose-dependent inhibiting effect of HU on DNA synthesis. Partial synchronization of the cells demonstrated by a distinct mitotic peak 10 h after HU application is not due to a G1/S block of the cells and their subsequent synchronous passage through the cycle after release from the block but is due to rather complex mechanisms of action of HU: a differential cytocidal effect and an effect on the passage of the cells through the cycle, both depending on the position of the cells throughout the cycle. HU kills S-phase cells, mainly cells in early S phase; i.e., a great portion of the cells "accumulated" in early S phase is killed by the drug, while G1-phase cells are almost not affected by the lethal effect of HU. These G1-phase cells pass through the cycle more rapidly after cessation of the HU effect. The same is true for the surviving cells accumulated in early S phase, while part of the cells in the remaining S phase are delayed in their passage through the cycle. This causes partial synchronization, since a great portion of all cells that survive HU treatment reach mitosis at the same time. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/3335224 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/0014482788901577/1-s2.0-0014482788901577-main.pdf?_tid=2f513bb7456d5604e612c2ee7f3bcd01&acdnat=1333701127_c277e511481dd03b1c38ceb815414595 | - |
| heal.journalName | Exp Cell Res | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1988 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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