The Value of Neuroendocrine Markers in Nonsmall Cell Lung-Cancer - a Comparative Immunohistopathologic Study
Φόρτωση...
Ημερομηνία
Συγγραφείς
Kiriakogianipsaropoulou, P.
Malamoumitsi, V.
Martinopoulou, U.
Legaki, S.
Tamvakis, N.
Vrettou, E.
Fountzilas, G.
Skarlos, D.
Kosmidis, P.
Pavlidis, N.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Lung Cancer
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
In order to estimate the value of immunohistochemical identification of neuroendocrine (NE) differentiation markers in non-small cell lung carcinomas (NSCLCs), we investigated the expression of five neuroendocrine and neural differentiation-related antigens in 51 NSCLCs. Additionally, 20 epithelial lung tumors with NE differentiation [15 carcinoids and five small cell lung carcinomas (SCLCs)] and 61 epithelial tumors of various other origin (breast, prostate, colon and head-neck carcinomas) were studied. An indirect two-stage immunoperoxidase method was performed in formalin-fixed and paraffin-embedded tissue specimens, by using commercially available monoclonal antibodies. These antibodies are directed against neuron-specific enolase (NSE), chromogranin-A and Leu-7 which are general markers of NE differentiation, bombesin, which is a specific NE secretory product and neurofilament triplet protein (NFTP), an intermediate filament protein of neuronal differentiation. All five markers demonstrated a positive immunoreactivity in NSCLCs, equally distributed to all three histologic subtypes, ranging from 16 to 47% of the cases (NSE 47%, bombesin 21.5%, Leu-7 21.5%, chromogranin-A 18% and NFTP 16%). Most of the carcinoids and SCLCs expressed multiple or all NE markers. The other four epithelial tumors showed a positive immunoreactivity for bombesin, Leu-7 and NFTP, ranging from 11 to 40% of the cases. Chromogranin-A was not expressed in any of these tumors, whereas NSE was demonstrated only in 17% of breast carcinomas. The following remarks can be drawn from this study: (1) some NSCLCs showed immunophenotypic NE differentiation; (2) among all the markers used, NSE was the most sensitive (sensitivity, 100%) and chromogranin-a the most specific (specificity, 100%); and (3) NSE and chromogranin-A appear to be the most valuable and useful indicators of probable neuroendocrine differentiation in lung epithelial tumors.
Περιγραφή
Λέξεις-κλειδιά
nsclc, neuroendocrine markers, nse, nftp, leu-7, bombesin, chromogranin-a, neuron-specific enolase, l-dopa decarboxylase, endocrine differentiation, chromogranin-a, tumors, adenocarcinoma, carcinoma, expression, antibodies, prognosis
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://A1994QN21600002
http://ac.els-cdn.com/0169500294921644/1-s2.0-0169500294921644-main.pdf?_tid=cdf00f93e120b72df9a44f6809646742&acdnat=1334232104_2fab096428f7781907b9ca5365666805
http://ac.els-cdn.com/0169500294921644/1-s2.0-0169500294921644-main.pdf?_tid=cdf00f93e120b72df9a44f6809646742&acdnat=1334232104_2fab096428f7781907b9ca5365666805
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών