Detection of mycobacteria in clinical specimen using the mycobacteria growth indicator tube (MGIT) and the Lowenstein Jensen medium

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dc.contributor.authorLevidiotou, S.en
dc.contributor.authorPapamichael, D.en
dc.contributor.authorGessouli, E.en
dc.contributor.authorGolegou, S.en
dc.contributor.authorAnagnostou, S.en
dc.contributor.authorGalanakis, E.en
dc.contributor.authorPapadopoulou, C.en
dc.contributor.authorAntoniadis, G.en
dc.date.accessioned2015-11-24T19:25:03Z
dc.date.available2015-11-24T19:25:03Z
dc.identifier.issn0944-5013-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22564
dc.rightsDefault Licence-
dc.subjectmycobacteriaen
dc.subjectmgiten
dc.subjectljen
dc.subjectsolid-cultureen
dc.subjectrecoveryen
dc.subjecttuberculosisen
dc.subjectsystemen
dc.subjectbactec-460en
dc.subjectratesen
dc.titleDetection of mycobacteria in clinical specimen using the mycobacteria growth indicator tube (MGIT) and the Lowenstein Jensen mediumen
heal.abstractThe recovery rates of mycobacteria strains isolated from 1200 clinical specimens using the mycobacteria growth indicator tube (MGIT) system and the conventional Lowenstein Jensen medium (LJ) were assessed. Of the 87 mycobacterial isolates recovered, 54 belonged to the M. tuberculosis complex (MTB) and 33 to the non-tuberculosis complex (NTM). MGIT recovered 78 (89.65%) mycobacteria isolates (51 MTB (94.44%) and 27 NTM (81.81%) and LJ recovered 70 (80.46%) mycobacteria isolates (49 MTB (90.74%) and 21 NTM (63.63%). Sixty one (70.1%) of the total mycobacteria isolates were recovered with both systems (46 (85.2%) MTB and 15 (45.5%) NTM. No significant difference was found between MGIT and LJ (p > 0.05) in both MTB and NTM recoveries. The average detection time for MTB was significantly shorter with MGIT than with LJ, in both the smear-positive specimens (8 vs 30 days: p < 0.0001) and smear-negative specimens (15 vs 30 days: p < 0.001). The average detection time of NTM was also shorter for MGIT (15 vs 30 days: p < 0.0001). However, the contamination rate was higher in MGIT (8.5%) than in LJ (3%). The results suggest that the use of MGIT contributes to a more rapid and effective diagnosis of mycobacterial infections particulary when combined with the classical LJ.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondary<Go to ISI>://000082996000008-
heal.journalNameMicrobiol Resen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1999-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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