Effect of ambient oxygen concentration on lipofuscin accumulation in cultured rat heart myocytes--a novel in vitro model of lipofuscinogenesis

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dc.contributor.authorSohal, R. S.en
dc.contributor.authorMarzabadi, M. R.en
dc.contributor.authorGalaris, D.en
dc.contributor.authorBrunk, U. T.en
dc.date.accessioned2015-11-24T19:23:06Z
dc.date.available2015-11-24T19:23:06Z
dc.identifier.issn0891-5849-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22246
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectCells, Cultureden
dc.subjectFemaleen
dc.subjectFluorescenceen
dc.subjectHeart/drug effectsen
dc.subjectLipofuscin/*metabolismen
dc.subjectMaleen
dc.subjectMicroscopy, Electronen
dc.subjectMyocardium/*metabolism/ultrastructureen
dc.subjectOrganelles/metabolismen
dc.subjectOxidation-Reductionen
dc.subjectOxygen/*pharmacologyen
dc.subjectPigments, Biological/*metabolismen
dc.subjectRatsen
dc.subjectRats, Inbred Strainsen
dc.subjectSpectrometry, Fluorescenceen
dc.titleEffect of ambient oxygen concentration on lipofuscin accumulation in cultured rat heart myocytes--a novel in vitro model of lipofuscinogenesisen
heal.abstractThe objective of this study was to elucidate the factors involved in the accumulation of lipofuscin in post-mitotic cells. The hypothesis that oxidative stress accelerates the rate of lipofuscin accumulation was tested by examining the effects of 5%, 20%, and 40% ambient oxygen concentration on lipofuscin content in cultured rat cardiac myocytes. Lipofuscin was quantified by microspectrofluorometry at 7 and 12 days of in vitro age. Lipofuscin-emitted yellow autofluorescence increased in direct relationship to ambient oxygen concentration with age. Transmission electron microscopic examination of the cells after 3, 8, and 12 days in culture indicated a progressive time and oxygen dependent increase in the frequency and size of lipofuscin organelles. The results are interpreted to suggest that oxidative stress is one of the causal factors in the accumulation of lipofuscin.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/2912825-
heal.identifier.secondaryhttp://www.sciencedirect.com/science/article/pii/089158498990155X-
heal.journalNameFree Radic Biol Meden
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1989-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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