Regulation of prothymosin alpha during the cell cycle

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dc.contributor.authorVareli, K.en
dc.contributor.authorTsolas, O.en
dc.contributor.authorFrangou-Lazaridis, M.en
dc.date.accessioned2015-11-24T18:54:37Z
dc.date.available2015-11-24T18:54:37Z
dc.identifier.issn0014-2956-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/18707
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectBase Sequenceen
dc.subject*Carrier Proteinsen
dc.subjectCell Cycle/*physiologyen
dc.subject*Cell Cycle Proteinsen
dc.subjectCell Division/physiologyen
dc.subjectCell Lineen
dc.subjectCulture Mediaen
dc.subjectCyclinsen
dc.subject*DNA-Binding Proteinsen
dc.subjectE2F Transcription Factorsen
dc.subjectG2 Phaseen
dc.subjectGene Expression Regulationen
dc.subjectHumansen
dc.subjectMolecular Sequence Dataen
dc.subjectPromoter Regions, Geneticen
dc.subjectProtein Precursors/*biosynthesis/genetics/immunologyen
dc.subjectRNA, Messenger/analysisen
dc.subjectRetinoblastoma-Binding Protein 1en
dc.subjectS Phaseen
dc.subjectThymosin/*analogs & derivatives/biosynthesis/genetics/immunologyen
dc.subjectTranscription Factor DP1en
dc.subjectTranscription Factors/geneticsen
dc.subjectTranscription, Geneticen
dc.titleRegulation of prothymosin alpha during the cell cycleen
heal.abstractA number of studies have indicated that the small nuclear acidic protein prothymosin alpha is associated with cellular-proliferation events. For example, c-myc causes immediate transcriptional activation of prothymosin alpha, and prothymosin alpha antisense oligonucleotides inhibit myeloma cell division. To investigate the regulation of prothymosin alpha, we examined its mRNA and protein levels during the cell cycle of mononuclear cells and fibroblastic cells. We isolated immunoreactive material from cellular extracts and immunolocalized the protein to the nucleus during the cell cycle. We reported here that the material present in the cells is prothymosin alpha rather than the amino-terminal peptide thymosin alpha 1. [3H]Thymidine-incorporation studies associate maximum accumulation of mRNA and protein with the S/G2 phase of the cell cycle. This induction of prothymosin alpha mRNA seems to resemble cyclin B expression and is more pronounced in fibroblasts. Moreover, transient-transfection experiments indicate that transcription factor E2F is a strong positive regulator of the prothymosin alpha gene. Our results are consistent with the hypothesis that prothymosin alpha is involved in proliferation checkpoints of the cell cycle.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/8706683-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1111/j.1432-1033.1996.0799w.x/asset/j.1432-1033.1996.0799w.x.pdf?v=1&t=h2lkt0iw&s=f23e0d9559fdc3ed5a3bb6ba0ef308f1bc52411b-
heal.journalNameEur J Biochemen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1996-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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