DNA binding selectivity of oligopyridine-ruthenium(II)-lysine conjugate
| dc.contributor.author | Triantafillidi, K. | en |
| dc.contributor.author | Karidi, K. | en |
| dc.contributor.author | Novakova, O. | en |
| dc.contributor.author | Malina, J. | en |
| dc.contributor.author | Garoufis, A. | en |
| dc.date.accessioned | 2015-11-24T16:56:27Z | |
| dc.date.available | 2015-11-24T16:56:27Z | |
| dc.identifier.issn | 1477-9226 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/10444 | |
| dc.rights | Default Licence | - |
| dc.subject | interstrand cross-links | en |
| dc.subject | cell-free media | en |
| dc.subject | deoxynucleotide duplex | en |
| dc.subject | enantioselective binding | en |
| dc.subject | supramolecular cylinder | en |
| dc.subject | antitumor complexes | en |
| dc.subject | anticancer drugs | en |
| dc.subject | arene complexes | en |
| dc.subject | phase-i | en |
| dc.subject | ruthenium | en |
| dc.title | DNA binding selectivity of oligopyridine-ruthenium(II)-lysine conjugate | en |
| heal.abstract | The synthesis, characterization and DNA binding properties of the complex [Ru(terpy)(4,4'-(COLysCONH(2))(2)bpy)Cl](3+) (1) have been studied. Complex (1) hydrolyzes to (2) with a calculated rate constant K(h) = 2.35 +/- 0.08 x 10(-4) s(-1) and binds coordinatively to ct-DNA, with a saturation r-value at about 0.1. Stabilization of the ct-DNA helix at low electrolyte (NaClO(4)) concentration (10 mM) and destabilization at higher electrolyte concentrations (50-200 mM) was observed. Circular dichroism studies indicate that the hydrolyzed complex binds to DNA, increasing the unwinding of the DNA helix with an unwinding angle calculated as Phi = 12 +/- 2 degrees. The positive LD signal observed at 350 nm indicates some kind of specificity in complex orientation towards the global DNA axis. Complex (2) binds specifically to G4 on the central part of the oligonucleotide duplexes d(CGCGCG)(2) and d(GTCGAC)(2), as evidenced by NMR spectroscopy. Both lysine moieties were found to interact most likely electrostatically with the DNA phosphates, assisting the coordinative binding and increasing the DNA affinity of the complex. Photoinduced DNA cleavage by (2), upon UVA irradiation was observed, but despite its relative high DNA affinity, it was incomplete (similar to 12%). | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | Doi 10.1039/C0dt00554a | - |
| heal.identifier.secondary | <Go to ISI>://000285419900022 | - |
| heal.identifier.secondary | http://pubs.rsc.org/en/content/articlepdf/2011/dt/c0dt00554a | - |
| heal.journalName | Dalton Transactions | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2011 | - |
| heal.publisher | Royal Society of Chemistry | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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