Differentiation of human retinoblastoma in vitro into cell types with characteristics observed in embryonal or mature retina

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dc.contributor.authorTsokos, M.en
dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorChader, G. J.en
dc.contributor.authorTriche, T. J.en
dc.date.accessioned2015-11-24T19:24:26Z
dc.date.available2015-11-24T19:24:26Z
dc.identifier.issn0002-9440-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22475
dc.rightsDefault Licence-
dc.subjectBucladesine/pharmacologyen
dc.subjectButyric Aciden
dc.subjectButyric Acids/pharmacologyen
dc.subjectCell Differentiationen
dc.subjectCells/classificationen
dc.subjectCells, Cultureden
dc.subjectCytological Techniquesen
dc.subjectCytoplasm/ultrastructureen
dc.subjectEye Neoplasms/*pathology/ultrastructureen
dc.subjectFluorescenceen
dc.subjectFormaldehyde/diagnostic useen
dc.subjectHistocytochemistryen
dc.subjectHumansen
dc.subjectImmunochemistryen
dc.subjectMicroscopy, Electronen
dc.subjectMicrovilli/ultrastructureen
dc.subjectNeuroglia/pathologyen
dc.subjectNeurons/pathologyen
dc.subjectRetina/*cytology/embryology/growth & developmenten
dc.subjectRetinoblastoma/*pathology/ultrastructureen
dc.subjectRosette Formationen
dc.titleDifferentiation of human retinoblastoma in vitro into cell types with characteristics observed in embryonal or mature retinaen
heal.abstractThe capacity of a primitive human retinoblastoma cell line (Y-79) to differentiate into several cell types of normal human retina was investigated. Cells were studied in suspension and monolayer cultures, in serum-free or serum-supplemented medium, and in the presence or absence of differentiating agents such as N6O12-dibutyryl adenosine 3',5'-cyclic monophosphate (dbc-AMP) and sodium butyrate (Nabut). Electron microscopy, immunohistochemistry for detection of myelin basic protein (MBP), and formaldehyde-induced fluorescence (FIF) for catecholamines were performed. Treated cells exhibited morphologic characteristics supportive of differentiation toward photoreceptors, conventional neurons and glial cells, increased FIF reactivity, and MBP expression. Growth in serum-free medium without differentiating agents led to a similar but less enhanced morphologic differentiation. These results confirm the concept that human retinoblastoma originates from a primitive neuroectodermal multipotential cell.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/3013012-
heal.journalNameAm J Patholen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1986-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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