In vivo generation of 3' and 5' truncated species in the process of c-myc mRNA decay
| dc.contributor.author | Ioannidis, P. | en |
| dc.contributor.author | Havredaki, M. | en |
| dc.contributor.author | Courtis, N. | en |
| dc.contributor.author | Trangas, T. | en |
| dc.date.accessioned | 2015-11-24T16:31:31Z | |
| dc.date.available | 2015-11-24T16:31:31Z | |
| dc.identifier.issn | 0305-1048 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7455 | |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | Gene Expression Regulation/*drug effects | en |
| dc.subject | *Genes, myc | en |
| dc.subject | HeLa Cells | en |
| dc.subject | Humans | en |
| dc.subject | Hydrolysis | en |
| dc.subject | Interferon-alpha/*pharmacology | en |
| dc.subject | Mice | en |
| dc.subject | RNA, Messenger/genetics/*metabolism | en |
| dc.title | In vivo generation of 3' and 5' truncated species in the process of c-myc mRNA decay | en |
| heal.abstract | It has been demonstrated that the half-life of c-myc mRNA is modulated in response to physiological agents. The elucidation of the decay process and the identification of the critical steps in the in vivo c-myc mRNA degradation pathway can be approached by following the fate of c-myc mRNA under the influence of such factors. IFN-alpha was the factor used to modulate c-myc mRNA half-life in HeLa 1C5 cells, a stable clone derived from HeLa cells. This cell line carries multiple copies of the c-myc gene, under the control of the dexamethasone inducible mouse mammary tumor virus-long terminal repeat (MMTV-LTR). Exposure of HeLa 1C5 cells to IFN-alpha resulted in a further 2-fold increase over the dexamethasone-induced c-myc mRNA. However, the c-myc mRNA in IFN-alpha treated cells was less stable than that in the control cells. RNase H mapping of the 3' untranslated region of c-myc mRNA revealed, in addition to the full length mRNA, three smaller fragments. These fragments were proven to be truncated, non-adenylated c-myc mRNA species generated in vivo. Exposure of HeLa 1C5 cells to Interferon-alpha before induction with dexamethasone resulted in the enhanced presence of these intermediates. RNase H analysis of c-myc mRNA after actinomycin D chase revealed that deadenylation led to the formation of a relatively more stable oligoadenylated c-myc mRNA population which did not appear to be precursor to the truncated intermediates. The detection of truncated 3' end c-myc mRNA adenylated fragments as well, implies that the c-myc mRNA degradation process may follow an alternative pathway possibly involving endonucleolytic cleavage. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/9016668 | - |
| heal.identifier.secondary | http://nar.oxfordjournals.org/content/24/24/4969.full.pdf | - |
| heal.journalName | Nucleic Acids Res | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1996 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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