Modulation of endothelial cell morphogenesis in vitro by MMP-9 during glial-endothelial cell interactions

Απλή σελίδα τεκμηρίου
dc.contributor.authorChandrasekar, N.en
dc.contributor.authorJasti, S.en
dc.contributor.authorAlfred-Yung, W. K.en
dc.contributor.authorAli-Osman, F.en
dc.contributor.authorDinh, D. H.en
dc.contributor.authorOlivero, W. C.en
dc.contributor.authorGujrati, M.en
dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorNicolson, G. L.en
dc.contributor.authorRao, J. S.en
dc.contributor.authorMohanam, S.en
dc.date.accessioned2015-11-24T19:04:08Z
dc.date.available2015-11-24T19:04:08Z
dc.identifier.issn0262-0898-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19981
dc.rightsDefault Licence-
dc.subjectBrain/*blood supplyen
dc.subjectCapillariesen
dc.subjectCarcinogens/pharmacologyen
dc.subjectCell Communication/*physiologyen
dc.subjectCells, Cultureden
dc.subjectEndothelium, Vascular/*cytologyen
dc.subjectHumansen
dc.subjectMatrix Metalloproteinase 9/*physiologyen
dc.subjectMicrocirculationen
dc.subjectNeovascularization, Physiologicen
dc.subjectNeuroglia/*physiologyen
dc.subjectProtein Kinase C/metabolismen
dc.subjectTetradecanoylphorbol Acetate/pharmacologyen
dc.subjectTissue Inhibitor of Metalloproteinase-1/*physiologyen
dc.titleModulation of endothelial cell morphogenesis in vitro by MMP-9 during glial-endothelial cell interactionsen
heal.abstractThe purpose of this study was to investigate the roles of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the formation of capillary structures by human brain microvascular endothelial cells cocultured with SNB19 glioblastoma cells. Unstimulated cocultures did not form capillaries and produce MMP-9 but stimulation with the protein kinase C (PKC) activator 4-phorbol-12-myristate 13-acetate (PMA) produced MMP-9 and capillary networks. Addition of recombinant MMP-9 increased capillary formation. Anti-MMP-9 antibodies, TIMP-1, the synthetic MMPs inhibitor Batimastat (BB-94), and the PKC inhibitor calphostin-C all reduced MMP-9 activity and capillary network formation in these cocultures. Cytochalasin-D in the presence of PMA suppressed MMP-9 expression and capillary formation, but colchicine-B had no such effect. Finally, PMA-induced MMP-9 expression and capillary formation were inhibited by the MEKK-specific inhibitor PD98059. These results suggest that MMP-9 is important in endothelial cell morphogenesis and the formation of capillaries in glial/endothelial cocultures in vitro.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/11448065-
heal.journalNameClin Exp Metastasisen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2000-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

Αρχεία

Φάκελος/Πακέτο αδειών

Προβολή: 1 - 1 of 1
Μικρογραφία εικόνας
Ονομα:
license.txt
Μέγεθος:
1.74 KB
Μορφότυπο:
Item-specific license agreed upon to submission
Περιγραφή:
Κατεβάστε

Συλλογές

Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ