1-O-alkyl-2-acetyl-sn-glycero-3-phosphate : phosphohydrolase activity in Tetrahymena pyriformis
Φόρτωση...
Ημερομηνία
Συγγραφείς
Tellis, C.
Lekka, M. E.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Wiley-Blackwell
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Eukaryotic Microbiology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Within the frame of the de novo formation of Platelet-Activating Factor in Tetrahymena, the occurrence as well as the properties of a lipid phosphate phosphohydrolase activity catalyzing the dephosphorylation of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphate was investigated. The activity was distributed in all the membrane fractions of the cell and in the cytosol. It showed preference for acyl-acetyl-sn-glycero-phosphate as well, and at a much lower level, for dipalmitoyl-glycero-phosphate. Mg2+ and Ca2+ caused a dose-dependent inhibition, while F-, EDTA and EGTA had no effect. The enzymic activity was linear for at least up to 60 min incubation time and up to 150 mu g protein. Microsomal activity exhibited two optimal pH areas, around 7.0 and 9.0, while mitochondrial activity showed one peak, at pH 7.0. Acyl-GP, acyl-acetyl-GP and alkyl-GP could replace alkyl-acetyl-GP in significant rates, while dipalmitoyl-GP, beta-GP, fructose-6-GP, p-nitrophenylphosphate, creatine phosphate or ATP had no effect. Side phospholipose A(2) and C activities were also detected. Taking into account the presence of PAF and alkylacetylglycerol in the protozoan as well as the presence of a dithiothreitol- insensitive CDP-choline:cholinephosphotransferase activity that converts alkylacetylglycerol to PAF, we suggest that the present phosphohdrolase activity may be involved in the de novo production of PAF within Tetrahymena.
Περιγραφή
Λέξεις-κλειδιά
alkyl-acetyl-glycerophosphate : phosphohydrolase paf, phosphatidate phosphohydrolase, phospholipase a(2), phospholipase c, phospholipid biosynthesis, platelet-activating-factor, phosphatidate phosphohydrolase, rat-liver, subcellular-distribution, biosynthesis, fractions, purification, yeast, cells, assay
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000086049500003
http://onlinelibrary.wiley.com/store/10.1111/j.1550-7408.2000.tb00021.x/asset/j.1550-7408.2000.tb00021.x.pdf?v=1&t=h0dxyth1&s=91ef864cd08d7f819f6c127e182f7f7a190bbed0
http://onlinelibrary.wiley.com/store/10.1111/j.1550-7408.2000.tb00021.x/asset/j.1550-7408.2000.tb00021.x.pdf?v=1&t=h0dxyth1&s=91ef864cd08d7f819f6c127e182f7f7a190bbed0
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας