A pentylenetetrazole-induced generalized seizure in early life enhances the efficacy of muscarinic receptor coupling to G-protein in hippocampus and neocortex of adult rat
Φόρτωση...
Ημερομηνία
Συγγραφείς
Potier, S.
Senecal, J.
Chabot, J. G.
Psarropoulou, C.
Descarries, L.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
European Journal of Neuroscience
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
We have previously shown that exposure to the anti-cholinesterase eserine provokes interictal-like discharges in the CA3 area of hippocampal slices from adult rats in which a generalized seizure has been induced by pentylenetetrazole (PTZ) when immature (at 20 days). Such increased responsiveness to acetylcholine (ACh) was not associated with any change in hippocampal acetylcholine or gamma-aminobutyric acid (GABA) content, GABAergic inhibition or density of ACh innervation, but was blocked by the muscarinic receptor antagonist atropine. We therefore turned to quantitative radioligand binding autoradiography, in situ hybridization and the [S-35]GTP gamma S method to assess the properties of hippocampal and neocortical muscarinic receptors in adult rats having experienced a PTZ seizure at P20. The densities of M1 and M2 receptor binding sites, respectively labeled with [H-3]pirenzepine and [H-3]AFDX-384, as well as the amount of m1, m2 and m3 receptor mRNAs, did not differ from control in the hippocampus and neocortex of these rats. In contrast, in PTZ rats, both brain regions displayed a marked increase in [S-35]GTP gamma S incorporation stimulated by ACh, bethanechol and particularly oxotremorine. This finding indicates that a generalized seizure in immature rat can entail a long-term and presumably permanent increase in the efficacy of G-protein coupling to muscarinic receptors in the hippocampus and neocortex of the adult. By analogy, such a mechanism could account for the susceptibility to epilepsy of human adults having suffered from prolonged convulsions in early life.
Περιγραφή
Λέξεις-κλειδιά
binding, epileptogenesis, g-protein coupling, mrna, muscarinic receptors, guinea-pig brain, binding-sites, messenger-rna, acetylcholine-receptors, neonatal seizures, regional expression, estrogen-treatment, cerebral-cortex, sex-differences, nervous-syst
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000228751800007
http://onlinelibrary.wiley.com/store/10.1111/j.1460-9568.2005.04026.x/asset/j.1460-9568.2005.04026.x.pdf?v=1&t=hfxnu12o&s=319c3c5085b7d99b7b0e9f4f9ac8adbf42d595bb
http://onlinelibrary.wiley.com/store/10.1111/j.1460-9568.2005.04026.x/asset/j.1460-9568.2005.04026.x.pdf?v=1&t=hfxnu12o&s=319c3c5085b7d99b7b0e9f4f9ac8adbf42d595bb
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών