v-FBR-fos oncogene fails to rescue mammalian cells from growth arrest but affects the responses of human fibroblasts to heparin
| dc.contributor.author | Kolettas, E. | en |
| dc.contributor.author | Evangelou, A. | en |
| dc.contributor.author | Gonos, E. S. | en |
| dc.date.accessioned | 2015-11-24T18:49:38Z | |
| dc.date.available | 2015-11-24T18:49:38Z | |
| dc.identifier.issn | 0250-7005 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/17988 | |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | *Cell Aging | en |
| dc.subject | Cell Division | en |
| dc.subject | Cell Line | en |
| dc.subject | Cell Line, Transformed | en |
| dc.subject | *Cell Transformation, Viral | en |
| dc.subject | Fibroblasts/*metabolism | en |
| dc.subject | Heparin/*pharmacology | en |
| dc.subject | Humans | en |
| dc.subject | Mesoderm/cytology | en |
| dc.subject | Oncogene Proteins v-fos/biosynthesis/genetics/*physiology | en |
| dc.subject | Proto-Oncogene Proteins c-jun/biosynthesis/genetics | en |
| dc.subject | RNA, Messenger/biosynthesis | en |
| dc.subject | Rats | en |
| dc.subject | *Sarcoma Viruses, Murine | en |
| dc.subject | Transfection | en |
| dc.title | v-FBR-fos oncogene fails to rescue mammalian cells from growth arrest but affects the responses of human fibroblasts to heparin | en |
| heal.abstract | The effects of v-fos oncogene on the proliferation of mammalian cells were studied using several approaches. Constitutive overexpression of v-FBR-fos in normal human fibroblasts (MRC-5) and of v-FBR-fos in human chondrocytes (HAC21) failed to immortalise them, extend their in vitro lifespan, increase their growth rates or induce cellular transformation. Further, v-FBR-fos did not render MRC-5 growth factor-independent or alter their responsivenness to serum, but it markedly suppressed their heparin-induced proliferation. A conditionally immortalized, temperature-sensitive rat embryo fibroblast cell line (tsa14) which undergoes growth arrest upon inactivation of a thermolabile SV40 large T antigen by a temperature shift producing a phenotype that mimmicks the senescent phenotype, was also used to study the effects of v-FBR-fos on cell proliferation. Whereas a wild-type SV40 large T antigen rescued tsa14 from a temperature-dependent growth arrest, v-FBR-fos failed to do so. Hence, v-FBR-fos was not sufficient to, at least, complement the tsa14 growth defect. There was no change in the expression of c-jun and junB, members of the AP-1 transcriptional complex in MRC-5v-fos cells. These data show that v-FBR-fos is not sufficient to rescue mammalian cells from senescence but it can affect the responses of human fibroblasts to heparin suggesting a role of fos in cell proliferation. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/11299775 | - |
| heal.journalName | Anticancer Res | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2001 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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