Core mutations in patients with acute episodes of chronic HBV infection are associated with the emergence of new immune recognition sites and the development of high IgM anti-HBc index values
Φόρτωση...
Ημερομηνία
Συγγραφείς
Alexopoulou, A.
Baltayiannis, G.
Eroglu, C.
Nastos, T.
Dourakis, S. P.
Archimandritis, A.
Karayiannis, P.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
J Med Virol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Acute exacerbations in HBeAg negative patients with chronic hepatitis B virus (HBV) infection are invariably associated with concurrent increases in the index of IgM class antibodies against the core protein (anti-HBc) of the virus. This study aimed to investigate whether this was related to the clearance of variants from the quasispecies pool and the appearance of new ones, with aminoacid substitutions in well recognized B-cell epitopes. In this study, 5 HBeAg negative patients (A to E) with 13 sequential serum samples (A1-A2, B1-B2-B3, C1-C2, D1-D2-D3, E1-E2-E3) were investigated after amplification of the entire core encoding region followed by cloning/sequencing studies. The sequences at different time points were compared with those from a single HBeAg positive patient with no apparent acute exacerbations. The results from sequence comparison showed that virus variants emerged in all (A2, B3, C2, D3, E2, and E3) but two (B2 and D2) subsequent sera with amino-acid substitutions affecting B-cell epitopes. It is concluded that the rise in the values of IgM anti-HBc may be attributed to the alteration of the antigenic epitopes leading to new antibody production in the majority of the cases. However, it appears that increases in IgM anti-HBc indexes in a few cases may relate to other possible mechanisms which are discussed.
Περιγραφή
Λέξεις-κλειδιά
Adult, DNA, Viral/chemistry/genetics, Epitopes/genetics/immunology, Female, Hepatitis B Antibodies/*blood, Hepatitis B Core Antigens/*genetics/*immunology, Hepatitis B virus/genetics/immunology, Hepatitis B, Chronic/*immunology/*virology, Humans, Immunoglobulin M/*blood, Longitudinal Studies, Male, Middle Aged, Mutation, Missense/*immunology, Sequence Analysis, DNA
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/19031457
http://onlinelibrary.wiley.com/store/10.1002/jmv.21337/asset/21337_ftp.pdf?v=1&t=h0uldfoj&s=8081678ae76cec2150951c014b4a8dd2b1b9c0f7
http://onlinelibrary.wiley.com/store/10.1002/jmv.21337/asset/21337_ftp.pdf?v=1&t=h0uldfoj&s=8081678ae76cec2150951c014b4a8dd2b1b9c0f7
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής