Purification and characterization of an extracellular alpha-L-arabinofuranosidase from Fusarium oxysporum
| dc.contributor.author | Christakopoulos, P. | en |
| dc.contributor.author | Katapodis, P. | en |
| dc.contributor.author | Hatzinikolaou, D. G. | en |
| dc.contributor.author | Kekos, D. | en |
| dc.contributor.author | Macris, B. J. | en |
| dc.date.accessioned | 2015-11-24T16:32:21Z | |
| dc.date.available | 2015-11-24T16:32:21Z | |
| dc.identifier.issn | 0273-2289 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7544 | |
| dc.rights | Default Licence | - |
| dc.subject | Chromatography, Gel | en |
| dc.subject | Chromatography, Ion Exchange | en |
| dc.subject | Chromatography, Thin Layer | en |
| dc.subject | Electrophoresis, Polyacrylamide Gel | en |
| dc.subject | Fusarium/*enzymology | en |
| dc.subject | Glycoside Hydrolases/*chemistry/*isolation & purification | en |
| dc.subject | Hydrogen-Ion Concentration | en |
| dc.subject | Temperature | en |
| dc.subject | Xylans/metaboli | en |
| dc.title | Purification and characterization of an extracellular alpha-L-arabinofuranosidase from Fusarium oxysporum | en |
| heal.abstract | An alpha-L-arabinofuranosidase from Fusarium oxysporum F3 was purified to homogeneity by a two-step ion exchange intercalated by a gel filtration chromatography. The enzyme had a molecular mass of 66 kDa and was optimally active at pH 6.0 and 60 degrees C. It hydrolyzed aryl alpha-L-arabinofuranosides and cleaved arabinosyl side chains from arabinoxylan and arabinan. There was a marked synergistic effect between the alpha-L-arabinofuranosidase and an endo-(1-->4)-beta-D-xylanase produced by F. oxysporum in the extensive hydrolysis of arabinoxylan. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/10949693 | - |
| heal.journalName | Appl Biochem Biotechnol | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2000 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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