The role of deoxyribonuclease I in amniotic fluid during pregnancy and labour
| dc.contributor.author | Lolis, D. E. | en |
| dc.contributor.author | Kalantaridou, S. N. | en |
| dc.contributor.author | Stefos, T. | en |
| dc.contributor.author | Makrydimas, G. | en |
| dc.contributor.author | Georgiou, I. | en |
| dc.date.accessioned | 2015-11-24T19:31:03Z | |
| dc.date.available | 2015-11-24T19:31:03Z | |
| dc.identifier.issn | 0301-2115 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23198 | |
| dc.rights | Default Licence | - |
| dc.subject | Amniotic Fluid/*enzymology | en |
| dc.subject | DNA/metabolism | en |
| dc.subject | Deoxyribonuclease I/*metabolism | en |
| dc.subject | Electrophoresis, Agar Gel | en |
| dc.subject | Female | en |
| dc.subject | Gestational Age | en |
| dc.subject | Humans | en |
| dc.subject | Labor, Obstetric/*metabolism | en |
| dc.subject | Meconium | en |
| dc.subject | Placenta/enzymology | en |
| dc.subject | Pregnancy/*metabolism | en |
| dc.subject | Reference Values | en |
| dc.title | The role of deoxyribonuclease I in amniotic fluid during pregnancy and labour | en |
| heal.abstract | The purpose of this study was to detect the presence, and determine the normal values and the significance of DNase I in the amniotic fluid and in human placentas. Fifty-one pregnant women at 16-22 weeks of gestation and 89 women in spontaneous labour at term were recruited to the study. DNase I activity was measured in amniotic fluid and cytoplasmic extracts from the fetal portion of placentas by using a spectrophotometric technique (DNA precipitation assay) and nucleic acid electrophoresis, following degradation of the DNA by the enzyme. DNase I activity was undetectable in the placental cytoplasmic extracts. In the second trimester of pregnancy DNase I activity was detectable in amniotic fluid (2.3+/-0.64x10(5) U/l). During labour DNase I activity was also detectable, but reduced levels were observed in the presence of clear amniotic fluid, compared to second trimester levels, (1.9+/-0.44x10(5) U/l, P<0.001), whereas higher levels were found in the presence of meconium-stained amniotic fluid compared to both second trimester and clear amniotic fluid at labour (11.4+/-4.1x10(5) U/l, P<0.001 and P<0.001, respectively). Three out of 17 fetuses with meconium-stained amniotic fluid (18%) developed perinatal infection. In conclusion, the detection of DNase I activity in the amniotic fluid of second and third trimester indicates a physiological role in human pregnancy. DNase I activity normally decreases at term, compared to second trimester levels, but increases significantly in the presence of meconium. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/9578275 | - |
| heal.journalName | Eur J Obstet Gynecol Reprod Biol | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1998 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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