Post-fertilization effects of chronic renal failure in male rats
Φόρτωση...
Ημερομηνία
Συγγραφείς
Dimitriadis, F.
Giannakis, D.
Giotitsas, N.
Parldalidis, N.
Baltogiannis, D.
Saito, M.
Watanabe, T.
Gratsias, S.
Zikopoulos, K.
Pashopoulos, M.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Int J Androl
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
We evaluated the potential for growth and intrauterine development of embryos generated from the fertilization of oocytes with spermatozoa recovered from animals with chronic renal failure (CRF). Group A included sham-operated rats (n = 28), group B1 involved CRF rats that had undergone erythropoietin plus bromocryptine treatment (n = 28), and group B2 included CRF rats that had received normal saline. Embryos derived from the in vitro fertilization of oocytes with spermatozoa recovered from rats of group A or group B1 or group B2 were transferred to female recipients. We induced CRF in a group of rats (group B; n = 56; the total kidney volume was reduced to one-sixth with two operations). One week after the second operation, the rats of group B were randomly divided into group B1 (they subsequently received bromocryptine plus erythropoietin) and group B2 (they received injections of saline). Nine weeks after the second operation, the fertility of each male rat was assessed by mating tests and in vitro fertilization of oocytes. The mean litter size was significantly smaller in the subpopulation of fertile animals in group B2 than in the fertile rats of group B1 and in the fertile rats of group B1 than in the fertile rats of group A. Per cent of transferred blastocysts that developed into alive offspring were significantly lower in group B2 than in group B1 and in group B1 than in group A. Epididymal spermatozoa demonstrated a significantly larger DNA-oxidative damage in group B2 than in group B1 and in group B1 than in group A. These findings demonstrate that sperm-DNA damage because of CRF development is accompanied by a defect in the development of embryos generated in vitro. We may suggest that bromocryptine and erythropoietin protecting sperm DNA from oxidative damage improve reproductive potential in rats with CRF.
Περιγραφή
Λέξεις-κλειδιά
Animals, Epididymis, Erythropoietin/*pharmacology, Female, Fertility, Fertilization/*physiology, Fertilization in Vitro, Kidney/pathology, Kidney Failure, Chronic/*physiopathology, Male, Oocytes/*drug effects, Rats, Rats, Wistar, Spermatozoa/*drug effects
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/19515174
http://onlinelibrary.wiley.com/store/10.1111/j.1365-2605.2008.00929.x/asset/j.1365-2605.2008.00929.x.pdf?v=1&t=h0kzq83t&s=ff1d8efd064f15e74a6e05684d414080f974c9e6
http://onlinelibrary.wiley.com/store/10.1111/j.1365-2605.2008.00929.x/asset/j.1365-2605.2008.00929.x.pdf?v=1&t=h0kzq83t&s=ff1d8efd064f15e74a6e05684d414080f974c9e6
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής