Distribution of Clostridium perfringens in polluted lake environments

dc.contributor.authorBezirtzoglou, E.en
dc.contributor.authorPanagiou, A.en
dc.contributor.authorSavvaidis, I.en
dc.contributor.authorMaipa, V.en
dc.date.accessioned2015-11-24T16:56:26Z
dc.date.available2015-11-24T16:56:26Z
dc.identifier.issn1075-9964-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/10441
dc.rightsDefault Licence-
dc.subjectclostridiumen
dc.subjectclostridium perfringensen
dc.subjectwateren
dc.subjectlakeen
dc.subjectphen
dc.subjectbacteriaen
dc.titleDistribution of Clostridium perfringens in polluted lake environmentsen
heal.abstractMany of the directives that relate to the prevention of pollution or the improvement of fresh water also relate to lake waters since lake waters ultimately inherit much of the pollution that enters into fresh water. In order to determine the influence of the water depth on Clostridium perfringens, we utilised a new medium, lactose-sulfite (LS) broth, suggested for rapid enumeration and identification of C, perfringens. Duplicate samples were collected at each one of the following sites of the polluted station: surface, 60cm, 90cm and bottom (1.18cm). Membrane filtration equipment was used. All samples were alternatively passed through two membrane filters, the first (20-25 mu m pore size) was used for retention of the abundant phytoplankton and the second (porosity 0.45 mu m) for C. perfringens. Membranes were placed into the first tube of ten-fold dilutions from 10(1) to 10(4) and incubated aerobically in a waterbath at 46 degrees C for 24h. The numbers of C. perfringens fluctuated depending on the water depth. Vegetative forms were found only in the bottom sampling; they were never found in surface, 60cm and 90cm sampling sites. Sporulated forms were found in all sampling sites with the exception of the surface sampling. Clostridium perfringens as an anaerobic bacterium never occurred in the surface waters in vegetative or spore forms, even if the waters were extremely polluted by domestic or industrial activities. Vegetative forms occurred only in the bottom samples but spore forms which are more resistant to various environmental effects occurred in all depths except for the surface. (C) 1997 Academic Press.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondary<Go to ISI>://A1997XW04900021-
heal.identifier.secondaryhttp://ac.els-cdn.com/S1075996497901013/1-s2.0-S1075996497901013-main.pdf?_tid=11e31f74d20f87195ba0e64f04adc36c&acdnat=1333040193_c29ea581eba01d0262f21e0778769d22-
heal.journalNameAnaerobeen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate1997-
heal.publisherAcademic Press Ltd.en
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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