Usefulness of Herpes Consensus PCR methodology to routine diagnostic testing for herpesviruses infections in clinical specimens
| dc.contributor.author | Vrioni, G. | en |
| dc.contributor.author | Kalogeropoulos, C. | en |
| dc.contributor.author | Gartzonika, C. | en |
| dc.contributor.author | Priavali, E. | en |
| dc.contributor.author | Levidiotou, S. | en |
| dc.date.accessioned | 2015-11-24T18:54:47Z | |
| dc.date.available | 2015-11-24T18:54:47Z | |
| dc.identifier.issn | 1743-422X | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/18743 | |
| dc.rights | Default Licence | - |
| dc.subject | Adult | en |
| dc.subject | Bodily Secretions/virology | en |
| dc.subject | Cerebrospinal Fluid/virology | en |
| dc.subject | Child | en |
| dc.subject | Cytomegalovirus/isolation & purification | en |
| dc.subject | DNA, Viral/genetics | en |
| dc.subject | Herpesviridae Infections/*diagnosis/*virology | en |
| dc.subject | Herpesvirus 1, Human/isolation & purification | en |
| dc.subject | Herpesvirus 2, Human/isolation & purification | en |
| dc.subject | Herpesvirus 3, Human/isolation & purification | en |
| dc.subject | Herpesvirus 4, Human/isolation & purification | en |
| dc.subject | Herpesvirus 6, Human/isolation & purification | en |
| dc.subject | Humans | en |
| dc.subject | Polymerase Chain Reaction/*methods | en |
| dc.subject | Skin/virology | en |
| dc.title | Usefulness of Herpes Consensus PCR methodology to routine diagnostic testing for herpesviruses infections in clinical specimens | en |
| heal.abstract | The purposes of the study were to assess the usefulness of simultaneously amplifying herpes simplex virus 1 and 2, varicella-zoster virus, cytomegalovirus, Epstein-Barr virus and human herpesvirus 6 DNA in various clinical specimens and to analyze clinical events in patients presenting positive results. A total of 763 clinical samples obtained from 758 patients, including 115 cerebrospinal fluids, 102 aqueous fluids, 445 swabs from genital (152), oro-facial (138) and other (155) skin lesions, 96 eye swabs and 5 bronchoalveolar lavages, were tested by using the Consensus polymerase chain reaction methodology. The clinical files of the patients were consulted retrospectively. 171 of the 758 patients (22.5%) were positive for at least one of the six target viruses: herpes simplex virus 1 (n = 95), varicella-zoster virus (n = 40), herpes simplex virus 2 (n = 21), herpes simplex virus 1 plus herpes simplex virus 2 (n = 8), cytomegalovirus (n = 4), Epstein-Barr virus (n = 1), human herpesvirus 6 (n = 1), and herpes simplex virus 1 plus human herpesvirus 6 (n = 1). The Consensus methodology enabled the rapid and accurate detection of herpesviruses in various clinical specimens and provided a reliable tool in the diagnosis of herpetic infections. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | 10.1186/1743-422X-4-59 | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/17562023 | - |
| heal.identifier.secondary | http://www.virologyj.com/content/pdf/1743-422X-4-59.pdf | - |
| heal.journalName | Virol J | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2007 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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