H-1 NMR determination of hypericin and pseudohypericin in complex natural mixtures by the use of strongly deshielded OH groups
| dc.contributor.author | Tatsis, E. C. | en |
| dc.contributor.author | Exarchou, V. | en |
| dc.contributor.author | Troganis, A. N. | en |
| dc.contributor.author | Gerothanassis, I. P. | en |
| dc.date.accessioned | 2015-11-24T16:38:34Z | |
| dc.date.available | 2015-11-24T16:38:34Z | |
| dc.identifier.issn | 0003-2670 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/8061 | |
| dc.rights | Default Licence | - |
| dc.subject | h-1 nuclear magnetic resonance | en |
| dc.subject | hypericin | en |
| dc.subject | pseudohypericin | en |
| dc.subject | hypericum perforatum | en |
| dc.subject | intramolecular hydrogen bond | en |
| dc.subject | peri hydroxyl groups | en |
| dc.subject | st-johns-wort | en |
| dc.subject | performance liquid-chromatography | en |
| dc.subject | magnetic-resonance-spectroscopy | en |
| dc.subject | intramolecular hydrogen-bonds | en |
| dc.subject | mass-spectrometry | en |
| dc.subject | nmr-spectroscopy | en |
| dc.subject | quantitative-analysis | en |
| dc.subject | qnmr spectroscopy | en |
| dc.subject | products analysis | en |
| dc.subject | chemical-shifts | en |
| dc.title | H-1 NMR determination of hypericin and pseudohypericin in complex natural mixtures by the use of strongly deshielded OH groups | en |
| heal.abstract | The H-1 NMR spectra of the commercially available compounds hypericin and its derivative pseudohypericin in CD3OH solutions indicate significantly deshielded signals in the region of 14-15 ppm. These resonances are attributed to the peri hydroxyl protons OH(6), OH(8) and OH(1), OH(13) of hypericins which participate in a strong six-membered ring intramolecular hydrogen bond with CO(7) and CO(14), respectively, and therefore, they are strongly deshielded. In the present work, we demonstrate that one-dimensional H-1 NMR spectra of hypericin and pseudohypericin, in Hypericum perforatum extracts show important differences in the chemical shifts of the hydroxyl groups with excellent resolution in the region of 14-15 ppm. The facile identification and quantification of hypericin and its derivative compound pseudohypericin was achieved, without prior HPLC separation, for two H. perforatum extracts from Greek cultivars and two commercial extracts: a dietary supplement, and an antidepressant medicine. The results were compared with those obtained from UV-vis and LC/MS measurements. (C) 2007 Elsevier B.V. All rights reserved. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | DOI 10.1016/j.aca.2007.11.040 | - |
| heal.identifier.secondary | <Go to ISI>://000252758000013 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0003267007019198/1-s2.0-S0003267007019198-main.pdf?_tid=fc84ff94-3246-11e3-ae54-00000aab0f01&acdnat=1381476801_902b8ffca195babcb001467d8bfe98cc | - |
| heal.journalName | Analytica Chimica Acta | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2008 | - |
| heal.publisher | Elsevier Masson | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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