Selective chromatographic fractionation of catechol estrogens on anion exchangers in borate form
| dc.contributor.author | Fotsis, T. | en |
| dc.contributor.author | Heikkinen, R. | en |
| dc.date.accessioned | 2015-11-24T18:52:38Z | |
| dc.date.available | 2015-11-24T18:52:38Z | |
| dc.identifier.issn | 0022-4731 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/18418 | |
| dc.rights | Default Licence | - |
| dc.subject | Anion Exchange Resins | en |
| dc.subject | Borates | en |
| dc.subject | Chromatography, Gas | en |
| dc.subject | Chromatography, Ion Exchange/methods | en |
| dc.subject | Estrogens, Catechol/*isolation & purification | en |
| dc.subject | Indicators and Reagents | en |
| dc.title | Selective chromatographic fractionation of catechol estrogens on anion exchangers in borate form | en |
| heal.abstract | The borate form of anion exchangers has been investigated for its utility in the field of estrogen analysis. The borate form of a weak (DEAE-Sephadex A-25) and a strong (QAE-Sephadex A-25) anion exchanger was easily prepared by appropriate washing of the gels, without the need of time consuming immobilization techniques. Estrogens with vicinal cis-hydroxyls were strongly retained in both gels through formation of borate complexes and readily separated from estrogens not possessing such groups. Moreover, borate complex formation with the labile o-dihydroxyphenyl moiety of catechol estrogens fully protected them from decomposition during chromatography. Quantitative recovery of catechol estrogens was thereby obtained without use of antioxidants. The borate form of QAE-Sephadex A-25 was capable, in addition, of separating estrogens not possessing vicinal cis-hydroxyls from the corresponding neutral steroids. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/6298506 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/0022473183901152/1-s2.0-0022473183901152-main.pdf?_tid=21df0e7b7ef6692b60df3894a4e07077&acdnat=1332751296_e3902d00e924b47259e97afbc10bfea2 | - |
| heal.journalName | J Steroid Biochem | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1983 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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