Highly constrained cyclic (S,S) -CXaaC- peptides as inhibitors of fibrinogen binding to platelets
Φόρτωση...
Ημερομηνία
Συγγραφείς
Kouki, A.
Mitsios, J. V.
Sakarellos-Daitsiotis, M.
Sakarellos, C.
Tselepis, A. D.
Tsikaris, V.
Tsoukatos, D. C.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Blackwell Publishing
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Thrombosis and Haemostasis
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The Arg-Gly-Asp RGD motif of adhesive proteins is recognized by the activated platelet integrin alpha(IIb)beta 3. Binding of fibrinogen (Fg) to activated alpha(IIb)beta(3) causes platelet aggregation and thrombus formation. Highly constraint cyclic (S,S) CXaaC- containing peptides incorporating the (S,S) -CDC- and (S,.S) -CRC- motifs were tested for their ability to inhibit platelet aggregation and Fg binding. Our results suggest that the above cyclic scaffolds stabilize a favorable structure for the antiaggregatory activity (IC50-values ranged from 1.7 to 570 pm). The peptides inhibited Fg binding with IC50-values up to 30-fold lower than those determined for the inhibition of the adenosine diphosphate (ADP)-induced platelet aggregation. Importantly, peptides (S,S) PSRCDCR-NH2 (peptide 11) and (S,S) PRCDCK-NH2 (peptide 10) did not inhibit PAC-1 binding to the activated platelets at a concentration in which they completely inhibited Fg binding. Moreover, (S,S) PSRCDCR-NH2 (peptide 11), one of the more active peptides, inhibited ADP-induced P-selectin exposure. By contrast, peptide (S,S) Ac-RWDCRC-NH2, incorporating the inverse (S,S)-DCRC-sequence (peptide 16), failed to inhibit P-selectin exposure whereas at the same concentration, it effectively inhibited PAG I and Fg binding. It is concluded that peptides containing the (S,S) -CDC- as well the (S,S) -CRC- sequences, exhibit a broad range of activities toward platelets, and could be helpful tools for elucidating the structural interaction of Fg with the integrin receptor alpha(IIb)beta(3), in its activated form. Furthermore, the (S,S) RCDC- sequence can be used as a scaffold for developing potent non-RGD-like Fg-binding inhibitors.
Περιγραφή
Λέξεις-κλειδιά
antiaggregatory agents, fibrinogen-binding inhibitors, integrin alpha(iib)beta(3) inhibitors, platelet activation inhibitors, glycoprotein-iib-iiia, iib/iiia antagonists, rgd peptides, integrin alpha(iib)beta(3), receptor, activation, adhesion, arg, alpha(iib)-subunit, alpha-v-beta-3
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000232443200027
http://onlinelibrary.wiley.com/store/10.1111/j.1538-7836.2005.01487.x/asset/j.1538-7836.2005.01487.x.pdf?v=1&t=h0e0mukc&s=bd858378361b7b854624f7bce6ae970e03459e0f
http://onlinelibrary.wiley.com/store/10.1111/j.1538-7836.2005.01487.x/asset/j.1538-7836.2005.01487.x.pdf?v=1&t=h0e0mukc&s=bd858378361b7b854624f7bce6ae970e03459e0f
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας