A modified method allows for correlation between NADPH-diaphorase histochemistry and immunohistochemistry for the demonstration of neuronal nitric oxide synthase (nNOS)

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dc.contributor.authorGrozdanovic, Z.en
dc.contributor.authorNakos, G.en
dc.contributor.authorMayer, B.en
dc.contributor.authorGossrau, R.en
dc.date.accessioned2015-11-24T19:40:14Z
dc.date.available2015-11-24T19:40:14Z
dc.identifier.issn0239-8508-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/24319
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectFemaleen
dc.subjectFluorescent Antibody Techniqueen
dc.subjectFormaldehydeen
dc.subjectGuinea Pigsen
dc.subjectHistocytochemistry/*methodsen
dc.subjectHistocytological Preparation Techniquesen
dc.subjectImmunohistochemistry/methodsen
dc.subjectMaleen
dc.subjectMiceen
dc.subjectNADPH Dehydrogenase/*metabolismen
dc.subjectNeurons/enzymologyen
dc.subjectNitric Oxide/*biosynthesisen
dc.subjectNitric Oxide Synthase/*analysisen
dc.subjectRatsen
dc.subjectRats, Wistaren
dc.subjectSubstrate Specificityen
dc.titleA modified method allows for correlation between NADPH-diaphorase histochemistry and immunohistochemistry for the demonstration of neuronal nitric oxide synthase (nNOS)en
heal.abstractResults obtained with the conventional nitro blue tetrazolium salt method for the visualization of the NADPH-diaphorase (NADPH-d) activity of nitric oxide synthase (NOS) are not specific for this particular enzyme, since this activity represents only a fraction of the total cellular NADPH-d pool. Therefore, the standard NADPH-d procedure was modified by performing the incubation in the presence of formaldehyde. Parallel application of the modified NADPH-d staining technique and the indirect immunofluorescence using an antibody against the neuronal isoenzyme (nNOS) on rat, mouse and guinea-pig tissues showed a correlation between histochemical and immunocytochemical staining. It can thus be concluded that the modified NADPH-d procedure allows for a more specific detection of the histochemical nNOS activity than the conventional method.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/7556767-
heal.journalNameFolia Histochem Cytobiolen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1995-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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