Determination of hydrogen peroxide by using a flow injection system with immobilized peroxidase and long pathlength capillary spectrophotometry
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Pappas, A. C.
Stalikas, C. D.
Fiamegos, Y. C.
Karayannis, M. I.
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Elsevier
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peer reviewed
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Analytica Chimica Acta
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The development of a highly sensitive method for the determination of nanomolar concentrations of hydrogen peroxide in the liquid phase is described. This paper demonstrates for the first time a flow injection analysis (FIA) system with immobilized enzyme reactor combined with a total internal reflective cell (a liquid waveguide capillary cell (LWCC)) and spectrophotometric detection, for the development of an improved procedure for the determination of hydrogen peroxide. Moreover, the newly synthesized 4-aminopyrazolone derivative, 4-amino-5-(p-aminophenyl)-1-methyl-2-phenyl-pyrazol-3-one (DAP), is used as a color coupler in its oxidative condensation with the sodium salt of N-ethyl-N-sulphopropylaniline sodium salt (ALPS) which acts as a hydrogen donor. Immobilization of peroxidase is achieved by coupling the periodate-treated enzyme to aminopropyl controlled-pore glass (CPG) beads. The determination of hydrogen peroxide is carried out in a 0.1 M phosphate buffer and the product is monitored at 590 nm with a charge-coupled device (CCD) detector equipped with fiber optics in a fully computerized system. The interference of different species, mainly ionic, was investigated. The method permits detection down to 4 nmol l(-1) hydrogen peroxide (signal-to-noise ratio = 3). A linear calibration graph was obtained over the range 20-700 nmol l(-1). The relative standard deviation (R.S.D.) at 300 nmol l(-1) H,02 is 1.7% (n = 7), The method was successfully applied for the determination of hydrogen peroxide in samples from a vat-cleaning process. (C) 2002 Elsevier Science B.V. All rights reserved.
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hydrogen peroxide, liquid waveguide capillary cell, immobilized peroxidase, flow injection analysis, amperometric determination, fluorometric-determination, horseradish-peroxidase, biosensor, water, chemiluminescence, electrode, reagent, samples, column
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<Go to ISI>://000174586200015
http://ac.els-cdn.com/S0003267001016002/1-s2.0-S0003267001016002-main.pdf?_tid=96d71ead45cd0ccc024441573e9df7a6&acdnat=1333040884_dafed7a350b247e1456128c4910c984a
http://ac.els-cdn.com/S0003267001016002/1-s2.0-S0003267001016002-main.pdf?_tid=96d71ead45cd0ccc024441573e9df7a6&acdnat=1333040884_dafed7a350b247e1456128c4910c984a
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en
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Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας