Regulation of catalytic behaviour of hydrolases through interactions with functionalized carbon-based nanomaterials
| dc.contributor.author | Pavlidis, I. V. | en |
| dc.contributor.author | Vorhaben, T. | en |
| dc.contributor.author | Gournis, D. | en |
| dc.contributor.author | Papadopoulos, G. K. | en |
| dc.contributor.author | Bornscheuer, U. T. | en |
| dc.contributor.author | Stamatis, H. | en |
| dc.date.accessioned | 2015-11-24T17:37:56Z | |
| dc.date.available | 2015-11-24T17:37:56Z | |
| dc.identifier.issn | 1388-0764 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/14421 | |
| dc.rights | Default Licence | - |
| dc.subject | lipase | en |
| dc.subject | esterase | en |
| dc.subject | interaction | en |
| dc.subject | carbon nanotubes | en |
| dc.subject | graphene oxide | en |
| dc.subject | enzyme immobilization | en |
| dc.subject | lipase immobilization | en |
| dc.subject | secondary structure | en |
| dc.subject | surface-chemistry | en |
| dc.subject | nanotubes | en |
| dc.subject | proteins | en |
| dc.subject | fluorescence | en |
| dc.subject | adsorption | en |
| dc.subject | reduction | en |
| dc.subject | stability | en |
| dc.title | Regulation of catalytic behaviour of hydrolases through interactions with functionalized carbon-based nanomaterials | en |
| heal.abstract | The interaction of enzymes with carbonbased nanomaterials (CBNs) is crucial for the function of biomolecules and therefore for the design and development of effective nanobiocatalytic systems. In this study, the effect of functionalized CBNs, such as graphene oxide (GO) and multi-wall carbon nanotubes (CNTs), on the catalytic behaviour of various hydrolases of biotechnological interest was monitored and the interactions between CBNs and proteins were investigated. The enzyme-nanomaterial interactions significantly affect the catalytic behaviour of enzymes, resulting in an increase up to 60 % of the catalytic efficiency of lipases and a decrease up to 30 % of the esterase. Moreover, the use of CNTs and GO derivatives, especially those that are amine-functionalized, led to increased thermal stability of most the hydrolases tested. Fluorescence and circular dichroism studies indicated that the altered catalytic behaviour of enzymes in the presence of CBNs arises from specific enzyme-nanomaterial interactions, which can lead to significant conformational changes. In the case of lipases, the conformational changes led to a more active and rigid structure, while in the case of esterases this led to destabilization and unfolding. Kinetic and spectroscopic studies indicated that the extent of the interactions between CBNs and hydrolases can be mainly controlled by the functionalization of nanomaterials than by their geometry. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | Doi 10.1007/S11051-012-0842-4 | - |
| heal.identifier.secondary | <Go to ISI>://000304155900008 | - |
| heal.journalName | Journal of Nanoparticle Research | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2012 | - |
| heal.publisher | Springer Netherlands | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Μηχανικών Επιστήμης Υλικών | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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