Intracellular oxygenation and cytochrome oxidase C activity in ischemic preconditioning of steatotic rabbit liver
Φόρτωση...
Ημερομηνία
Συγγραφείς
Hafez, T. S.
Glantzounis, G. K.
Fusai, G.
Taanman, J. W.
Wignarajah, P.
Parkes, H.
Fuller, B.
Davidson, B. R.
Seifalian, A. M.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Am J Surg
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
BACKGROUND: Mild to moderate steatotic livers are used as marginal donors in liver transplantation. Very little is known about the mechanisms of ischemia reperfusion (IR) injury (IRI) in fatty liver. This study aimed to establish whether cytochrome oxidase C (COX) activity is compromised by IRI in fatty liver and whether ischemic preconditioning (IPC) can protect COX activity. METHODS: New Zealand rabbits were fed on a high-cholesterol diet for 8 weeks to induce moderate hepatic steatosis. Three groups were tested. The IR group underwent 60 minutes of ischemia, followed by 7 hours of reperfusion. The IPC group (IPC + IR) underwent 5 minutes of ischemia, followed by 10 minutes of reperfusion and then 60 minutes of ischemia and 7 hours of reperfusion. The control group (sham) underwent the same surgical procedure, but ischemia was not induced. Deoxyhemoglobin, oxyhemoglobin, and change in the redox state of COX was continuously monitored in vivo by near-infrared spectroscopy. COX and citrate synthase (CS) activity assays were carried out on liver biopsy specimens in vitro. Bile was collected continuously during the procedure and analyzed using proton nuclear magnetic resonance spectroscopy. RESULTS: The IR group had decreased COX activity and tissue oxygenation represented by deoxyhemoglobin, oxyhemoglobin, COX, and elevated redox ratios of lactate/pyruvate and beta-hydroxybutarate/acetoacetate in vivo and a decrease in COX and CS activity in vitro. The IPC + IR group showed higher levels of all measured parameters in vivo and showed a smaller decrease in COX and CS activity in vitro. CONCLUSION: This study shows that IRI affects COX activity in fatty livers. This is attenuated by IPC.
Περιγραφή
Λέξεις-κλειδιά
Animals, Disease Models, Animal, Electron Transport Complex IV/*metabolism, Fatty Liver/*metabolism/pathology, Hemoglobins/metabolism, Intracellular Fluid/*metabolism, Ischemic Preconditioning/*methods, Oxygen Consumption/*physiology, Oxyhemoglobins/metabolism, Prognosis, Rabbits, Reperfusion Injury/metabolism/*prevention & control, Severity of Illness Index
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/20409534
http://ac.els-cdn.com/S0002961010000632/1-s2.0-S0002961010000632-main.pdf?_tid=e4ec74c8bf002a6c69f65fcc7f89dd5f&acdnat=1333626296_e77a00f7b7551d3f330d2cb71a92d3b8
http://ac.els-cdn.com/S0002961010000632/1-s2.0-S0002961010000632-main.pdf?_tid=e4ec74c8bf002a6c69f65fcc7f89dd5f&acdnat=1333626296_e77a00f7b7551d3f330d2cb71a92d3b8
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής