Presence of Epstein-Barr virus harbouring small and intermediate-sized cells in Hodgkin's disease. Is there a relationship with Reed-Sternberg cells?

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dc.contributor.authorJiwa, N. M.en
dc.contributor.authorKanavaros, P.en
dc.contributor.authorDe Bruin, P. C.en
dc.contributor.authorvan der Valk, P.en
dc.contributor.authorHorstman, A.en
dc.contributor.authorVos, W.en
dc.contributor.authorMullink, H.en
dc.contributor.authorWalboomers, J. M.en
dc.contributor.authorMeijer, C. J.en
dc.date.accessioned2015-11-24T18:56:57Z
dc.date.available2015-11-24T18:56:57Z
dc.identifier.issn0022-3417-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19115
dc.rightsDefault Licence-
dc.subjectDNA, Viral/analysisen
dc.subjectHerpesvirus 4, Human/genetics/*isolation & purificationen
dc.subjectHodgkin Disease/*microbiologyen
dc.subjectHumansen
dc.subjectImmunohistochemistryen
dc.subjectIn Situ Hybridizationen
dc.subjectPolymerase Chain Reactionen
dc.subjectReed-Sternberg Cells/*microbiologyen
dc.titlePresence of Epstein-Barr virus harbouring small and intermediate-sized cells in Hodgkin's disease. Is there a relationship with Reed-Sternberg cells?en
heal.abstractForty-four cases of Hodgkin's disease (HD), mostly of the nodular sclerosing type, were investigated for the presence of Epstein-Barr virus (EBV) by polymerase chain reaction (PCR) and DNA and RNA in situ hybridization (DISH, RISH), as well as by immunohistochemistry for the detection of latent membrane protein-1 (LMP-1) of EBV. In situ hybridization (ISH) was combined with immunohistochemistry to correlate the presence and activity of the virus at the cellular level. In 18/34 (53 per cent) cases, EBV-DNA sequences could be detected with the PCR method. In 12/18 positive cases, DISH and RISH were also positive. In the remaining six EBV-PCR positive cases, two were also positive with RISH and LMP-1, whereas no positive signal with DISH could be obtained. All DISH and/or RISH positive cases were also positive for LMP-1. With RISH, not only the Reed-Sternberg cells and their mononuclear variants (RS cells) stained positive, but also small and intermediate cells frequently reacted with the EBV-specific probes (EBER-1 and -2). Double staining with cellular markers (CD3, CD20, CD45, CD45RO, CD68, and the lectin PNA) revealed that most of the smaller EBER-positive cells frequently did not express T, B, or histiocytic markers, but that they, as well as the RS cells, showed cytoplasmic and membranous staining with PNA. These smaller EBER-positive cells were not found in EBV-PCR negative HD. EBER-positive RS cells were almost always LMP-1 positive, as well as a substantial proportion of the intermediate-sized cells, whereas the majority of the small EBER-positive cells remained LMP-1 negative.(ABSTRACT TRUNCATED AT 250 WORDS)en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1002/path.1711700206-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/8393921-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1002/path.1711700206/asset/1711700206_ftp.pdf?v=1&t=h0ez8dnr&s=81ab9d16238fcdef2388822f15478cfb0777831d-
heal.journalNameJ Patholen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1993-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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