An in vivo model of allergic inflammation: Pulmonary human cell infiltrate in allergen-challenged allergic Hu-SCID mice
Loading...
Date
Authors
Duez, C.
Tsicopoulos, A.
Janin, A.
TillieLeblond, I.
Thyphronitis, G.
Marquillies, P.
Hamid, Q.
Wallaert, B.
Tonnel, A. B.
Pestel, J.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Type
Type of the conference item
Journal type
peer reviewed
Educational material type
Conference Name
Journal name
Eur J Immunol
Book name
Book series
Book edition
Alternative title / Subtitle
Description
CB.17 severe combined immunodeficient (SCID) mice were used to establish a model of allergic pulmonary inflammation. SCID mice were intraperitoneally reconstituted with 10(7) peripheral blood mononuclear cells (PBMC) from patients sensitive to Dermatophagoides pteronyssinus (Dpt) and 2 weeks later were exposed to Dpt aerosols. After Dpt nebulization, SCID mice engrafted with PBMC from Dpt-sensitive patients developed a specific human ISE response as well as an intense pulmonary infiltrate of human cells. In contrast, SCID mice reconstituted with PBMC from patients allergic to grass pollen or from non-allegic donors failed to produce IgE or to exhibit a similar inflammatory response. The level of the IgE production was dependent on the IgE level of the allergic donor In the lungs of the same allergic SCID mice, 2 months after Dpt inhalation, the cell infiltrate contained CD45(+), CD45RO(-), CD20(+) and HLA-DR(+) human cells. They were located in perivascular and peribronchial areas and disseminated in the mouse lung parenchyma. Moreover mRNA IL-5(+) cells and eosinophils were found in peribronchial infiltrates. The observations indicate that humanized allergic SCID mice may develop, after nebulization with the relevant allergen, immune reactions similar to those observed in man and suggest that SCID mice may represent a useful model to analyze the regulatory mechanisms of IgE-associated allergic diseases.
Description
Keywords
allergy, inhalation, scid mouse, inflammation, severe combined immunodeficiency, blood mononuclear-cells, human peripheral-blood, messenger-rna, atopic subjects, immune-system, mast-cells, t-cells, induction, asthma
Subject classification
Citation
Link
<Go to ISI>://A1996UT53100019
Language
en
Publishing department/division
Advisor name
Examining committee
General Description / Additional Comments
Institution and School/Department of submitter
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών