A novel lipolytic activity of Rhodotorula glutinis cells: production, partial characterization and application in the synthesis of esters
| dc.contributor.author | Hatzinikolaou, D. G. | en |
| dc.contributor.author | Kourentzi, E. | en |
| dc.contributor.author | Stamatis, H. | en |
| dc.contributor.author | Christakopoulos, P. | en |
| dc.contributor.author | Kolisis, F. N. | en |
| dc.contributor.author | Kekos, D. | en |
| dc.contributor.author | Macris, B. J. | en |
| dc.date.accessioned | 2015-11-24T16:32:10Z | |
| dc.date.available | 2015-11-24T16:32:10Z | |
| dc.identifier.issn | 1389-1723 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7524 | |
| dc.rights | Default Licence | - |
| dc.title | A novel lipolytic activity of Rhodotorula glutinis cells: production, partial characterization and application in the synthesis of esters | en |
| heal.abstract | Cell-bound lipase activity (10 pNPL units/g dry cell weight) was released when the yeast Rhodotorula glutinis was cultured in a 7-l stirred tank fermentor using palm-oil as the sole carbon source. The enzyme showed relative specificity towards medium chain organic acids since the apparent K(m) values for pNPB (p-NitroPhenyl-Butyrate) and pNPL (p-NitroPhenyl-Laurate) were equal to 2.7 and 0.7 mM, respectively. In addition, 80% of this activity could be detected on the surface of the cells. The cell-bound nature of the enzyme increased its thermal stability showing half-life times of 200 and 60 min at 50 and 60 degrees C, respectively, as well as good stability in organic solvents. Freeze-dried cell preparations were successfully used to catalyze the synthesis of fatty acid esters of butanol and heptanol in nearly anhydrous organic solvents. A conversion of 60-62% was obtained upon esterification of palmitic or oleic acid with butanol, within 96 h. The enzyme preparation was used in four consecutive batch reactions with only 10% loss of activity. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/16232573 | - |
| heal.journalName | J Biosci Bioeng | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1999 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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