Proteins bound to heterogeneous nuclear RNA of simian-virus-40-infected cells
| dc.contributor.author | Pagoulatos, G. N. | en |
| dc.contributor.author | Yaniv, M. | en |
| dc.date.accessioned | 2015-11-24T18:55:54Z | |
| dc.date.available | 2015-11-24T18:55:54Z | |
| dc.identifier.issn | 0014-2956 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/18950 | |
| dc.rights | Default Licence | - |
| dc.subject | Base Sequence | en |
| dc.subject | Cell Line | en |
| dc.subject | Cell Transformation, Viral | en |
| dc.subject | Nucleic Acid Hybridization | en |
| dc.subject | *Nucleoproteins/biosynthesis | en |
| dc.subject | Protein Binding | en |
| dc.subject | *RNA, Heterogeneous Nuclear/metabolism | en |
| dc.subject | *RNA, Viral/metabolism | en |
| dc.subject | *Simian virus 40 | en |
| dc.subject | Viral Proteins/biosynthesis | en |
| dc.title | Proteins bound to heterogeneous nuclear RNA of simian-virus-40-infected cells | en |
| heal.abstract | Heterogeneous nuclear RNA . protein (hnRNA . protein) complexes from simian-virus-40 (SV40)-infected cells late in infection contain 7--10% RNA sequences specific to SV40 DNA. The SV40 nuclear RNA . protein complexes sediment at 60--70 S. The reality and specificity of the RNA-protein association is shown in metrizamide gradients. Protein and RNA lebels of hnRNA . protein-particles in SV40-infected cells follow a parallel pattern with a peak at 1.28 g/cm2 whereas a mixture of ribosomal RNA and soluble cytoplasmic proteins is separated according to the different densities in metrizamide. Analysis of hnRNA . protein from infected cells by two-dimensional gel electrophoresis shows the presence of a number of new proteins. It is demonstrated that three of these proteins are cellular ones induced by the virus infection and hence constitute good candidates to be specific RNA . protein particles for virus nuclear RNA. The presence of actin in hnRNA . protein particles from normal and SV40-infected cells and the presence of the major capsid protein VP1 in hnRNA . protein particles from SV40-infected cells is discussed. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/214301 | - |
| heal.identifier.secondary | http://onlinelibrary.wiley.com/store/10.1111/j.1432-1033.1978.tb20930.x/asset/j.1432-1033.1978.tb20930.x.pdf?v=1&t=h2bp560g&s=0a9206f6afa0ebc54459e49d5d66e40e7e83cff5 | - |
| heal.journalName | Eur J Biochem | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1978 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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