Interferon induces the interaction of prothymosin-alpha with STAT3 and results in the nuclear translocation of the complex
Φόρτωση...
Ημερομηνία
Συγγραφείς
Yang, C. H.
Murti, A.
Baker, S. J.
Frangou-Lazaridis, M.
Vartapetian, A. B.
Murti, K. G.
Pfeffer, L. M.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Exp Cell Res
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Interferons (IFNs) play critical roles in host defense by modulating the expression of various genes via tyrosine phosphorylation of STAT transcription factors. Many cytokines including IFNs induce tyrosine phosphorylation of the STAT3 transcription factor, which regulates acute phase gene expression. Using the yeast two-hybrid interaction trap, in which a tyrosine kinase is introduced into the yeast to allow tyrosine phosphorylation of bait proteins, prothymosin-alpha (ProTalpha) was identified to interact with the amino terminal half of tyrosine-phosphorylated STAT3. ProTalpha is a small, acidic, extremely abundant, and essential protein that may play a role in chromatin remodeling, and has been implicated in regulating the growth and survival of mammalian cells. Besides the interaction of tyrosine-phosphorylated STAT3 with ProTalpha in yeast cells, IFN induced the interaction of ProTalpha with STAT3 in mammalian cells, and this interaction was dependent on the tyrosine phosphorylation of STAT3. Moreover, IFNalpha induces the translocation of STAT3 and ProTalpha from the cytoplasm to the nucleus where these proteins colocalize. Since ProTalpha has an extremely strong nuclear localization and STAT proteins apparently lack any nuclear localization signals, the association of STAT3 with ProTalpha may provide a mechanism to result in STAT localization in the nucleus.
Περιγραφή
Λέξεις-κλειδιά
Active Transport, Cell Nucleus/drug effects/physiology, Animals, COS Cells, Cell Nucleus/drug effects/*metabolism, DNA-Binding Proteins/*metabolism, Interferon-alpha/pharmacology, Interferons/pharmacology/*physiology, Macromolecular Substances, Phosphorylation, Protein Precursors/*metabolism, Protein Structure, Tertiary/physiology, Protein Transport/drug effects/physiology, STAT3 Transcription Factor, Thymosin/*analogs & derivatives/*metabolism, Trans-Activators/*metabolism, Two-Hybrid System Techniques, Tyrosine/metabolism, Yeasts/metabolism
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/15242774
http://ac.els-cdn.com/S0014482704001934/1-s2.0-S0014482704001934-main.pdf?_tid=4ecf146dfd11521e639fc903f755fccd&acdnat=1332915128_733f32e22a6d885a3fc73bfd9ee2951e
http://ac.els-cdn.com/S0014482704001934/1-s2.0-S0014482704001934-main.pdf?_tid=4ecf146dfd11521e639fc903f755fccd&acdnat=1332915128_733f32e22a6d885a3fc73bfd9ee2951e
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής