Kinetics of Interleukin-4 Induction and Interferon-Gamma Inhibition of Ige Secretion by Epstein-Barr Virus-Infected Human Peripheral-Blood B-Cells

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dc.contributor.authorThyphronitis, G.en
dc.contributor.authorBanchereau, J.en
dc.contributor.authorHeusser, C.en
dc.contributor.authorTsokos, G. C.en
dc.contributor.authorLevine, A. D.en
dc.contributor.authorFinkelman, F. D.en
dc.date.accessioned2015-11-24T16:34:12Z
dc.date.available2015-11-24T16:34:12Z
dc.identifier.issn0008-8749-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7772
dc.rightsDefault Licence-
dc.subjectstimulatory factor-ien
dc.subjecthuman lymphocytes-ben
dc.subjectgrowth-factoren
dc.subjectcyto-toxicityen
dc.subjectmast-cellsen
dc.subjectactivationen
dc.subjectexpressionen
dc.subjectreceptoren
dc.subjectantibodyen
dc.subjectigg1en
dc.titleKinetics of Interleukin-4 Induction and Interferon-Gamma Inhibition of Ige Secretion by Epstein-Barr Virus-Infected Human Peripheral-Blood B-Cellsen
heal.abstractInterleukin-4 (IL-4) acts directly on purified human peripheral blood B cells cultured in the presence of Epstein-Barr virus (EBV) to induce IgE secretion and to enhance the secretion of IgG and IgM. Interferon-gamma (IFN-gamma) inhibits IgE secretion in this system, without affecting the secretion of the other Ig isotypes. To identify the time period during which EBV-infected B cells can be induced by IL-4 to secrete IgE, we have studied the effects of delayed addition of IL-4, or the termination of IL-4 stimulation by wash out or by neutralization with anti-IL-4 antibodies, on the induction of an IgE response. To induce a maximal IgE response, IL-4 had to be added to cultures of B cells plus EBV no later than 2 days after the initiation of culture, and had to remain present through the tenth day of culture. These two time points correspond to the initiation of detectable DNA synthesis (Days 3 to 4) and the earliest detectable Ig secretion (Days 10 to 12) by EBV-stimulated B cells. No IgE response was induced if the period during which EBV-stimulated B cells were cultured with IL-4 was less than 4 days, or if IL-4 were added later than the tenth day of culture, regardless of how long the culture was continued beyond that time. In contrast, IL-4 considerably enhanced IgG and IgM secretion and B cell CD23 expression, even if it was added after the tenth day of culture. IFN-gamma strongly inhibited the IgE response of B cells cultured with IL-4 plus EBV if added within 6 days of the initiation of culture, but had little effect on the generation of IgM or IgG responses made by these cells, regardless of the time of addition. Neither IL-4 nor IFN-gamma affected ongoing IgE secretion by an established, IgE-secreting, EBV-transformed cell line. These observations suggest that: (i) IL-4 first becomes able to induce EBV-activated B cells to secrete IgE as these cells begin to synthesize DNA, must stimulate B cells for at least 4 days to induce IgE secretion, and loses its ability to induce IgE secretion as these cells differentiate into Ig-secreting cells; (ii) the ability of IFN-gamma to suppress an IgE response is limited to this same time period; and (iii) IL-4 enhancement of CD23 expression and IgM and IgG secretion are independent of IL-4 induction of an IgE response.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondary<Go to ISI>://A1991FA01100012-
heal.journalNameCell Immunolen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate1991-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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