Treatment of MDS patients with recombinant human erythropoietin and the role of GSTs

dc.contributor.authorTsabouri, S. E.en
dc.contributor.authorGeorgiou, I.en
dc.contributor.authorKatsaraki, A.en
dc.contributor.authorBourantas, K. L.en
dc.date.accessioned2015-11-24T19:06:11Z
dc.date.available2015-11-24T19:06:11Z
dc.identifier.issn0392-9078-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/20310
dc.rightsDefault Licence-
dc.subjectAgeden
dc.subjectAged, 80 and overen
dc.subjectAllelesen
dc.subjectBlood Platelets/cytologyen
dc.subjectBlood Transfusionen
dc.subjectBone Marrow Cells/cytologyen
dc.subjectDNA/metabolismen
dc.subjectErythrocytes/cytologyen
dc.subjectErythropoietin/blood/*therapeutic useen
dc.subjectFemaleen
dc.subjectGene Deletionen
dc.subjectGenotypeen
dc.subjectGlutathione Transferase/genetics/*metabolismen
dc.subjectHemoglobins/metabolismen
dc.subjectHomozygoteen
dc.subjectHumansen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectMyelodysplastic Syndromes/*genetics/*therapyen
dc.subjectOdds Ratioen
dc.subjectPolymerase Chain Reactionen
dc.subjectPolymorphism, Geneticen
dc.subjectRecombinant Proteins/*therapeutic useen
dc.subjectTime Factorsen
dc.titleTreatment of MDS patients with recombinant human erythropoietin and the role of GSTsen
heal.abstractGlutathione S-transferases (GSTs) are a group of enzymes involved in the detoxification process of carcinogens and other substances. The genes encoding isoenzymes M1 and T1 have "null" alleles, which are polymorphic in humans. Our purpose was to examine whether the GSTM1 and GSTT1 homozygous null genotypes have an impact on the response to recombinant human erythropoietin (rhuEpo) treatment in MDS patients. We analyzed lymphocyte DNA samples from 27 patients with all types of myelodysplastic syndromes (MDS) at the time of diagnosis. All patients were scheduled to receive rHuEpo in doses of 150 u/Kg/day for a period of 12 weeks in order to obtain and maintain stable responses. A multiplex polymerase chain reaction (PCR) was used to genotype both GSTM1 and GSTT1 simultaneously, in responders and non-responders to rhuEpo with respect to various pretreatment parameters: haemoglobin, white blood cell count, platelets, serum erythropoietin, transfusion requirements and bone marrow blasts. The data obtained were evaluated by chi2 test and odds ratio were extracted. Twelve out of 27 evaluated patients demonstrated an erythroid response (44%). Nine out of the 12 patients (75%) responding after 12 weeks of treatment had GSTM1 null genotype (OR=3.4). In contrast, only 1 responder (8.3%) was homozygotes of GSTT1 null genotype. Furthermore, no statistically significant difference in the response rate of the different MDS subgroups was observed. Our results suggest that a treatment with rHuEpo may be effective in achieving a stable erythroid response in MDS patients who carry an homozygous deletion of the GSTM1 gene.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/15595630-
heal.journalNameJ Exp Clin Cancer Resen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2004-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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