Immunohistochemical expression of the p53, mdm2, p21/Waf-1, Rb, p16, Ki67, cyclin D1, cyclin A and cyclin B1 proteins and apoptotic index in T-cell lymphomas

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dc.contributor.authorKanavaros, P.en
dc.contributor.authorBai, M.en
dc.contributor.authorStefanaki, K.en
dc.contributor.authorPoussias, G.en
dc.contributor.authorRontogianni, D.en
dc.contributor.authorZioga, E.en
dc.contributor.authorGorgoulis, V.en
dc.contributor.authorAgnantis, N. J.en
dc.date.accessioned2015-11-24T19:11:54Z
dc.date.available2015-11-24T19:11:54Z
dc.identifier.issn0213-3911-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/20978
dc.rightsDefault Licence-
dc.subject*Adenovirus E1A Proteinsen
dc.subjectApoptosis/*immunologyen
dc.subjectCarrier Proteins/*analysisen
dc.subjectCell Cycle Proteins/analysisen
dc.subjectCyclin A/analysisen
dc.subjectCyclin B/analysisen
dc.subjectCyclin B1en
dc.subjectCyclin D1/analysisen
dc.subjectCyclin-Dependent Kinase Inhibitor p16/*analysisen
dc.subjectCyclin-Dependent Kinase Inhibitor p21en
dc.subjectCyclins/*analysisen
dc.subjectHumansen
dc.subjectImmunohistochemistryen
dc.subjectIn Situ Nick-End Labelingen
dc.subjectKi-67 Antigen/*analysisen
dc.subjectLymphoma, T-Cell, Peripheral/*metabolismen
dc.subjectNeoplasm Proteins/analysisen
dc.subject*Nuclear Proteinsen
dc.subjectPrecursor Cell Lymphoblastic Leukemia-Lymphoma/*metabolismen
dc.subjectProto-Oncogene Proteins/*analysisen
dc.subjectProto-Oncogene Proteins c-mdm2en
dc.subjectRepressor Proteinsen
dc.subjectStatistics as Topicen
dc.subjectTumor Suppressor Protein p53/*analysisen
dc.titleImmunohistochemical expression of the p53, mdm2, p21/Waf-1, Rb, p16, Ki67, cyclin D1, cyclin A and cyclin B1 proteins and apoptotic index in T-cell lymphomasen
heal.abstractFifty-seven cases of T-cell lymphomas (TCL) including 5 lymphoblastic (T-LBL) and 52 peripheral TCL (PTCL) were analyzed by immunohistochemistry for the expression of p53, mdm2, p21, Rb, cyclin D1, cyclin A, cyclin B1, and Ki67/MIB1 proteins and 39/52 PTCL were also analyzed for the expression of p16 protein and for the presence of apoptotic cells by the TUNEL method. The aim was to search for abnormal immunoprofiles of p53 and Rb growth control pathways and to determine the proliferative activity and the apoptotic index of TCL. Abnormal overexpression of p53, p21 and mdm2, in comparison to normal lymph nodes, was found in 12/57, 10/57 and 2/57 cases of TCL, respectively. Abnormal loss of Rb and p16 expression was found in 1/57 and 2/39 cases, respectively, whereas abnormal overexpression of cyclin D1 was not detected in any of the 57 cases. Our data revealed entity-related p53/p21/mdm2 phenotypes. Indeed, most nodal and cutaneous CD30+ anaplastic large cell lymphomas (ALCL) showed concomitant overexpression of p53 and p21 proteins (7/8 cases), and mdm2 was overexpressed in 2 p53-positive nodal ALCL. In contrast, overexpression of p53 was found in 3/17 cases of nodal peripheral TCL unspecified (PTCL-UC) and 2/7 non-ALCL cutaneous pleomorphic TCL. Overexpression of p21 protein was detected in 2/3 p53-positive PTCL-UC and in 1/2 p53-positive non-ALCL cutaneous pleomorphic TCL. Finally, all the remaining 25 cases of TCL did not show p53 and p21 overexpression. Overall, the p53+/p21+ phenotype in 10/57 TCL suggests wild-type p53 capable of inducing p21 expression. The highest apoptotic index (AI) was found in ALCL and a positive correlation between apoptotic index and Ki67 index (p<0.001) was detected. Ki67, cyclin A and cyclin B1 expression was found in all 57 TCL and on the basis of the combined use of these 3 variables, 3 groups of proliferative activity could be determined: a) high in ALCL and T-LBL, b) low in mycosis fungoides (MF) and gammadelta hepatosplenic TCL, and c) intermediate in the remaining TCL entities. The proliferative activity in the 12 p53 overexpressing cases was higher in comparison to the 45 p53-negative cases. Ki67 expresion in more than 25% of tumour cells showed significant correlation with p53 overexpression (p<0.001). Rb expression tended to be parallel to Ki67, cyclin A and cyclin B1 expression in all but one case of nodal PTCL-UC which displayed loss of RB expression. Interestingly, this case was p53-negative, whereas the p53-positive cases were Rb-positive. These findings suggest that different pathogenetic routes may function in some TCL, involving either the p53 or, less frequently, the Rb pathways.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/11332692-
heal.journalNameHistol Histopatholen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2001-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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