Use of ionic liquids as media for the biocatalytic preparation of flavonoid derivatives with antioxidant potency
Φόρτωση...
Ημερομηνία
Συγγραφείς
Katsoura, M. H.
Polydera, A. C.
Tsironis, L.
Tselepis, A. D.
Stamatis, H.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Biotechnology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Biocatalytic preparation of acylated derivatives of flavonoid glycosides was performed using various immobilized lipases in two different ionic liquids, namely 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) and 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim]PF6). The influence of various reaction parameters on the performance and the regioselectivity of the biocatalytic process was pointed out, using as model reaction the acylation of naringin and rutin with vinyl butyrate, catalyzed by immobilized Candida antarctica lipase at 60 degrees C. The biocatalytic modification of flavonoids strongly depended on the ionic liquid used, the molar ratio of substrates, as well as the acyl donor chain length. The highest conversion yield (about 65% after 96 h of incubation) was obtained with short chain acyl donors (up to four carbon atoms), at a relatively high molar ratio (10-15) in both ionic liquids used. The amount of monoacylated flavonoid derivatives produced in a single-step biocatalytic process in [bmim]BF4 was up to 5.5 g/L for monoacylated rutin and 30 g/L for monoacylated naringin. The regioselectivity of the process was higher in [bmim]BF4 than in [bmim]PF6 or organic solvents. Reaction rates observed in ionic liquids were up to four times higher than those reported for organic media. The acylation of sugar moiety of rutin with various acyl donors affected its antioxidant potential towards both isolated LDL and total serum model in vitro. A significant increase of antioxidant activity was observed for rutin-4"'-O-oleate. (c) 2006 Elsevier B.V. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
lipase, ionic liquids, flavonoids, biocatalysis, ldl oxidation, antioxidants, low-density-lipoprotein, candida-antarctica lipase, regioselective acylation, enzymatic-synthesis, carbon-dioxide, natural compounds, organic-solvents, phenolic-acids, ldl oxi
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000238102500010
http://ac.els-cdn.com/S0168165606000101/1-s2.0-S0168165606000101-main.pdf?_tid=d92fa2dde0c8cf371d14892d27d8c0ba&acdnat=1335510894_304e6d9a5d2d579127a9c0750a07d42a
http://ac.els-cdn.com/S0168165606000101/1-s2.0-S0168165606000101-main.pdf?_tid=d92fa2dde0c8cf371d14892d27d8c0ba&acdnat=1335510894_304e6d9a5d2d579127a9c0750a07d42a
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών