Ozone monitoring based on a biosensor concept utilizing a reagentless alcohol oxidase electrode
| dc.contributor.author | Stergiou, D. V. | en |
| dc.contributor.author | Prodromidis, M. I. | en |
| dc.contributor.author | Veltsistas, P. G. | en |
| dc.contributor.author | Evmiridis, N. P. | en |
| dc.date.accessioned | 2015-11-24T16:43:47Z | |
| dc.date.available | 2015-11-24T16:43:47Z | |
| dc.identifier.issn | 0003-2700 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/8738 | |
| dc.rights | Default Licence | - |
| dc.subject | flow-injection analysis | en |
| dc.subject | amperometric biosensors | en |
| dc.subject | formaldehyde | en |
| dc.subject | air | en |
| dc.subject | sensor | en |
| dc.subject | water | en |
| dc.subject | stoichiometry | en |
| dc.subject | acid | en |
| dc.subject | o-3 | en |
| dc.subject | ph | en |
| dc.title | Ozone monitoring based on a biosensor concept utilizing a reagentless alcohol oxidase electrode | en |
| heal.abstract | An electrochemical method based on the concept of a biosensor for the monitoring of ozone is described for first time. The proposed method includes two parts: a selective sorbent for ozone, that is, eugenol, and a formaldehyde amperometric biosensor mounted into a flowthrough cell. Ozone adds rapidly to the double bond of the allyl group of eugenol, which has been immobilized onto a hydrophobic C-18 reactor and the so produced formaldehyde is collected into the working buffer solution ( sampler) and pumped to the detector. A multimembrane assembly consisting of an alcohol oxidase-modified nylon membrane sandwiched between an outer polycarbonate and an inner cellulose acetate membrane was fitted onto a Pt electrode and the enzymatically produced H2O2 was monitored at + 0.65 V ( vs Ag/AgCl/ KCl 3 M). Under optimum conditions, a linear calibration curve over the concentration range 3-200 mu g.mL(-1) ozone was constructed. The detection limit (S/N = 3) was calculated at 1.1 mu g.mL(-1) ozone. The proposed method is interferencefree from other gases such as O-2, Ar, N-2, N2O, NOCl, SO2, NH3, and CO2, which were tested at concentrations > 200-fold higher than that of 100 mu g.mL(-1) ozone used for comparison. Besides selectivity, the method is easy to perform and reproducible; its applicability in synthetic gaseous samples is also demonstrated. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | Doi 10.1021/Ac060230h | - |
| heal.identifier.secondary | <Go to ISI>://000238665200059 | - |
| heal.identifier.secondary | http://pubs.acs.org/doi/pdfplus/10.1021/ac060230h | - |
| heal.journalName | Anal Chem | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2006 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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